Myt 1 Antibody (G-11): sc-74523
- Myt 1 Antibody (G-11) is a mouse monoclonal IgG1 κ Myt 1 antibody provided at 200 µg/ml
- raised against amino acids 1-304 mapping at the N-terminus of Myt 1 of human origin
- Myt 1 Antibody (G-11) is recommended for detection of Myt 1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Myt 1 Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Myt 1 (G-11): sc-74523.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Myt 1 Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Myt 1 Antibody (G-11) (see ordering information below).
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Myt 1 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Myt 1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Myt 1 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myt 1 protein plays a crucial role in cell cycle regulation, specifically in the phosphorylation of Cdc2, a key protein that controls the transition from G2 phase to mitosis. Myt 1 phosphorylates Cdc2 on threonine 14 and tyrosine 15, which is essential for keeping Cdc2 in an inactive state during S and G2 phases of the cell cycle. This regulation is vital for ensuring proper cell division and preventing premature mitotic entry, which could lead to genomic instability. Myt 1 activity is tightly controlled throughout the cell cycle, and Myt 1 interaction with other proteins, such as Wee 1 kinase, highlights Myt 1′s importance in the mitotic control network. Dephosphorylation of Cdc2 by Cdc25 in late G2 phase activates the Cdc2/cyclin B complex, allowing cells to progress into mitosis. Myt 1 (G-11) monoclonal antibody serves as a valuable tool for studying the dynamics of cell cycle regulation and mechanisms underlying mitotic control in human cells.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Myt 1 Antibody (G-11) References:
- Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein. | Strausfeld, U., et al. 1991. Nature. 351: 242-5. PMID: 1828290
- Wee1(+)-like gene in human cells. | Igarashi, M., et al. 1991. Nature. 353: 80-3. PMID: 1840647
- Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites. | Krek, W. and Nigg, EA. 1991. EMBO J. 10: 305-16. PMID: 1846803
- cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2. | Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
- Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. | Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
- Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. | Watanabe, N., et al. 1995. EMBO J. 14: 1878-91. PMID: 7743995
- The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex. | Liu, F., et al. 1997. Mol Cell Biol. 17: 571-83. PMID: 9001210
- Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15. | McGowan, CH. and Russell, P. 1993. EMBO J. 12: 75-85. PMID: 8428596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myt 1 Antibody (G-11) | sc-74523 | 200 µg/ml | $316.00 | |||
Myt 1 Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-527037 | 200 µg Ab; 10 µg BP | $354.00 | |||
Myt 1 Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-532410 | 200 µg Ab; 20 µg BP | $354.00 | |||
Myt 1 Antibody (G-11) AC | sc-74523 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Myt 1 Antibody (G-11) HRP | sc-74523 HRP | 200 µg/ml | $316.00 | |||
Myt 1 Antibody (G-11) FITC | sc-74523 FITC | 200 µg/ml | $330.00 | |||
Myt 1 Antibody (G-11) PE | sc-74523 PE | 200 µg/ml | $343.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 488 | sc-74523 AF488 | 200 µg/ml | $357.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 546 | sc-74523 AF546 | 200 µg/ml | $357.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 594 | sc-74523 AF594 | 200 µg/ml | $357.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 647 | sc-74523 AF647 | 200 µg/ml | $357.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 680 | sc-74523 AF680 | 200 µg/ml | $357.00 | |||
Myt 1 Antibody (G-11) Alexa Fluor® 790 | sc-74523 AF790 | 200 µg/ml | $357.00 |