PI 3-kinase p55γ Antibody (E-9): sc-376615

PI 3-kinase p55γ Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PI 3-kinase p55 gamma of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PI 3-kinase p55γ (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PI 3-kinase p55 gamma plays a crucial role in cellular signaling pathways, particularly in regulating cell growth, proliferation, and survival, making PI 3-kinase p55 gamma a significant target for cancer research and therapeutic interventions. PI 3-kinase p55 gamma is primarily located in the cytoplasm and translocates to the plasma membrane upon activation, where PI 3-kinase p55 gamma interacts with phosphatidylinositol 4,5-bisphosphate to initiate downstream signaling cascades. Proper localization of PI 3-kinase p55 gamma is essential for function, as mislocalization can lead to aberrant signaling and contribute to various diseases, including cancer. Human PI 3-kinase p55 gamma consists of a unique amino terminal region, a proline-rich motif, and two Src homology 2 (SH2) domains, which are critical for interaction with other signaling molecules. Research indicates that PI 3-kinase p55 gamma interacts with insulin-like growth factor-I receptor and insulin receptor, suggesting involvement in PI 3-kinase activation by these receptors.

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PI 3-kinase p55γ Antibody (E-9) References:

1. Multiple forms of p55PIK, a regulatory subunit of phosphoinositide 3-kinase, are generated by alternative initiation of translation.  |  Xia, X. and Serrero, G. 1999. Biochem J. 341 (Pt 3): 831-7. PMID: 10417350
2. The N-terminal 24 amino acids of the p55 gamma regulatory subunit of phosphoinositide 3-kinase binds Rb and induces cell cycle arrest.  |  Xia, X., et al. 2003. Mol Cell Biol. 23: 1717-25. PMID: 12588990
3. SOX4 expression in bladder carcinoma: clinical aspects and in vitro functional characterization.  |  Aaboe, M., et al. 2006. Cancer Res. 66: 3434-42. PMID: 16585165
4. Identification of IGF2 signaling through phosphoinositide-3-kinase regulatory subunit 3 as a growth-promoting axis in glioblastoma.  |  Soroceanu, L., et al. 2007. Proc Natl Acad Sci U S A. 104: 3466-71. PMID: 17360667
5. SREBP-1 regulates the expression of heme oxygenase 1 and the phosphatidylinositol-3 kinase regulatory subunit p55 gamma.  |  Kallin, A., et al. 2007. J Lipid Res. 48: 1628-36. PMID: 17452746
6. Integrative genomic analysis of phosphatidylinositol 3'-kinase family identifies PIK3R3 as a potential therapeutic target in epithelial ovarian cancer.  |  Zhang, L., et al. 2007. Clin Cancer Res. 13: 5314-21. PMID: 17875760
7. [Inhibitory effect of N-terminal 24 amino acids of the p55 gamma, a regulatory subunit of phosphoinositide 3-kinase, on proliferation of colon carcinoma cell line HT29].  |  Wang, GH., et al. 2008. Ai Zheng. 27: 1034-8. PMID: 18851781
8. BMP2-induced chemotaxis requires PI3K p55γ/p110α-dependent phosphatidylinositol (3,4,5)-triphosphate production and LL5β recruitment at the cytocortex.  |  Hiepen, C., et al. 2014. BMC Biol. 12: 43. PMID: 24885555
9. p55γ functional mimetic peptide N24 blocks vascular proliferative disorders.  |  Guo, J., et al. 2015. J Mol Med (Berl). 93: 1107-18. PMID: 25894383
10. Role of the p55-gamma subunit of PI3K in ALK-induced cell migration: RNAi-based selection of cell migration regulators.  |  Seo, M., et al. 2017. Cell Adh Migr. 11: 205-210. PMID: 27322022
11. Cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor.  |  Dey, BR., et al. 1998. Gene. 209: 175-83. PMID: 9524259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PI 3-kinase p55γ Antibody (E-9) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-PI 3-kinase p55γ Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PI 3-kinase p55γ Antibody (E-9) Specifications

Alternate Product Name: PI3K p55 gamma

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 333-380

Epitope Target: PI 3-kinase p55γ

Epitope Species: human

Product Reactivity/Detection: PI 3-kinase p55γ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for PI 3-kinase p55γ Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin