PDPK1 Antibody (A-10): sc-17766

PDPK1 Antibody (A-10) is a mouse monoclonal IgG1 antibody that detects PDPK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDPK1, also known as 3-phosphoinositide dependent protein kinase 1, is a 556 amino acid protein that is ubiquitously expressed and localizes to the cell membrane, cytoplasm, and nucleus, playing a crucial role in various cellular processes. PDPK1 (A-10) monoclonal antibody is essential for studying phosphorylation and activation of a subgroup of the AGC family of protein kinases, which are pivotal in mediating signal transduction pathways that control cell proliferation, survival, and growth, particularly in developing pancreatic beta cells. Additionally, PDPK1 regulates calcium uptake and calcium-activated potassium channels in mast cells, influences chemotaxis and motility in vascular endothelial cells, and is vital for cardiac homeostasis and thymocyte development. PDPK1′s structure includes a PH domain that is critical for homodimerization, localization, and nuclear import, alongside a protein kinase domain that facilitates enzymatic activity. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3, highlighting complexity and importance in cellular signaling networks. PDPK1 (A-10) monoclonal antibody is an invaluable tool for researchers studying these intricate pathways and diverse roles of PDPK1 in health and disease.

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PDPK1 Antibody (A-10) References:

1. Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma.  |  Chinen, Y., et al. 2014. Cancer Res. 74: 7418-29. PMID: 25269480
2. Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II.  |  Al-Zeer, MA., et al. 2017. EBioMedicine. 23: 100-110. PMID: 28803120
3. Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.  |  Li, D., et al. 2018. BMC Cancer. 18: 772. PMID: 30064387
4. LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p.  |  Hu, T., et al. 2020. Biomed Res Int. 2020: 4351671. PMID: 32025520
5. Rosmarinic Acid Regulates Microglial M1/M2 Polarization via the PDPK1/Akt/HIF Pathway Under Conditions of Neuroinflammation.  |  Wei, Y., et al. 2021. Inflammation. 44: 129-147. PMID: 32940818
6. Possible involvement of the Hedgehog and PDPK1-Akt pathways in the growth and migration of small-cell lung cancer.  |  Tang, N., et al. 2021. J Int Med Res. 49: 3000605211016562. PMID: 34038205
7. SUMOylation of PDPK1 Is required to maintain glycolysis-dependent CD4 T-cell homeostasis.  |  Sun, F., et al. 2022. Cell Death Dis. 13: 181. PMID: 35210408
8. A Genome-Wide Screen Identifies PDPK1 as a Target to Enhance the Efficacy of MEK1/2 Inhibitors in NRAS Mutant Melanoma.  |  Cai, W., et al. 2022. Cancer Res. 82: 2625-2639. PMID: 35657206
9. Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination.  |  Yang, M. and Li, L. 2024. Chem Biol Drug Des. 103: e14438. PMID: 38230783
10. Estrogen regulates PDPK1 to promote cell proliferation in epithelial ovarian cancer.  |  Wang, Y., et al. 2024. Heliyon. 10: e40296. PMID: 39624293

The preferred PDPK1 antibody is PDPK1 Antibody (E-3): sc-17765.