PDPK1 Antibody (E-3): sc-17765

PDPK1 Antibody (E-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDPK1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PDPK1 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. PDPK1, also known as 3-phosphoinositide dependent protein kinase 1, is a 556 amino acid protein that is ubiquitously expressed and primarily localized to the cell membrane, cytoplasm, and nucleus. As a master kinase, PDPK1 plays a crucial role in phosphorylating and activating a specific subgroup of the AGC family of protein kinases, which are essential for various cellular processes. PDPK1 is vital for mediating signal transduction pathways that control the proliferation, survival, and growth of developing pancreatic beta cells, as well as regulating calcium uptake and calcium-activated potassium channels in mast cells. Additionally, PDPK1 is involved in the regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 features a PH domain that is critical for homodimerization, localization, and nuclear import, alongside a protein kinase domain. The gene encoding PDPK1 is located on human chromosome 16p13.3 and gives rise to five alternatively spliced isoforms, highlighting the complexity and regulatory potential of this important protein in cellular signaling.

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PDPK1 Antibody (E-3) References:

1. PDK1, the master regulator of AGC kinase signal transduction.  |  Mora, A., et al. 2004. Semin Cell Dev Biol. 15: 161-70. PMID: 15209375
2. Novel small molecule inhibitors of 3-phosphoinositide-dependent kinase-1.  |  Feldman, RI., et al. 2005. J Biol Chem. 280: 19867-74. PMID: 15772071
3. Ablation of PDK1 in pancreatic beta cells induces diabetes as a result of loss of beta cell mass.  |  Hashimoto, N., et al. 2006. Nat Genet. 38: 589-93. PMID: 16642023
4. Role of the PH domain in regulating in vitro autophosphorylation events required for reconstitution of PDK1 catalytic activity.  |  Gao, X. and Harris, TK. 2006. Bioorg Chem. 34: 200-23. PMID: 16780920
5. Essential role of PDK1 in regulating endothelial cell migration.  |  Primo, L., et al. 2007. J Cell Biol. 176: 1035-47. PMID: 17371830
6. PDK1 regulates chemotaxis in human neutrophils.  |  Yagi, M., et al. 2009. J Dent Res. 88: 1119-24. PMID: 19892919
7. Ubiquitin-specific protease 4 inhibits mono-ubiquitination of the master growth factor signaling kinase PDK1.  |  Uras, IZ., et al. 2012. PLoS One. 7: e31003. PMID: 22347420
8. Phosphoinositide-dependent Kinase-1 (PDPK1) regulates serum/glucocorticoid-regulated Kinase 3 (SGK3) for prostate cancer cell survival.  |  Nalairndran, G., et al. 2020. J Cell Mol Med. 24: 12188-12198. PMID: 32926495
9. SCFFBXW5-mediated degradation of AQP3 suppresses autophagic cell death through the PDPK1-AKT-MTOR axis in hepatocellular carcinoma cells.  |  Liang, Y., et al. 2024. Autophagy. 20: 1984-1999. PMID: 38726865
10. Coronavirus M protein promotes mitophagy over virophagy by recruiting PDPK1 to phosphorylate SQSTM1 at T138.  |  Li, Y., et al. 2024. Nat Commun. 15: 8927. PMID: 39414765
11. 3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.  |  Alessi, DR., et al. 1997. Curr Biol. 7: 776-89. PMID: 9368760
12. Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B.  |  Stephens, L., et al. 1998. Science. 279: 710-4. PMID: 9445477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDPK1 Antibody (E-3) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-PDPK1 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDPK1 Antibody (E-3) Specifications

Alternate Product Name: PDPK1

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 229-556

Epitope Target: PDPK1

Epitope Species: human

Product Reactivity/Detection: PDPK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for PDPK1 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin