PKC Antibody (A-9): sc-17804
- PKC Antibody (A-9) is a mouse monoclonal IgG2a κ, cited in 16 publications, provided at 200 µg/ml
- raised against amino acids 373-672 mapping at the C-terminus of PKC of human origin
- Anti-PKC Antibody (A-9) is recommended for detection of all PKC family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PKC Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC (A-9): sc-17804.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PKC Antibody (A-9) for WB and IHC(P) applications. These reagents are now offered in bundles with PKC Antibody (A-9) (see ordering information below).
PKC Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects protein kinase C (PKC) across mouse, rat, and human species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PKC antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The protein kinase C family is crucial for regulating cellular functions, including cell growth, differentiation, gene expression, hormone secretion, and membrane dynamics. PKC proteins are primarily located in the cytoplasm and move to the cell membrane upon activation, playing a vital role in signal transduction pathways. This translocation activates downstream signaling cascades that influence various physiological processes. The PKC family includes conventional and novel isoforms, each showing distinct regulatory mechanisms and tissue-specific expression patterns. Understanding PKC localization and activation is key for revealing its role in health and disease, especially in cancer and other conditions where signaling pathways are disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKC Antibody (A-9) References:
- A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. | Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
- Protein kinase C (PKC) family in cancer progression. | Koivunen, J., et al. 2006. Cancer Lett. 235: 1-10. PMID: 15907369
- Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. | Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
- Expression and characterization of protein kinase C-delta. | Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
- PKC downregulation upon rapamycin treatment attenuates mitochondrial disease. | Martin-Perez, M., et al. 2020. Nat Metab. 2: 1472-1481. PMID: 33324011
- Targeted disruption of PKC from AKAP signaling complexes. | Limaye, AJ., et al. 2021. RSC Chem Biol. 2: 1227-1231. PMID: 34458835
- PKC-independent PI3K signalling diminishes PKC inhibitor sensitivity in uveal melanoma. | Park, JJ., et al. 2024. Oncogenesis. 13: 9. PMID: 38418838
- Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. | Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
- Turnover of inositol phospholipids and signal transduction. | Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
- The role of protein kinase C in cell surface signal transduction and tumour promotion. | Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
- Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. | Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
- Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. | Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC Antibody (A-9) | sc-17804 | 200 µg/ml | $316.00 | |||
PKC Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-525488 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKC Antibody (A-9): m-IgG2a BP-HRP Bundle | sc-546283 | 200 µg Ab; 10 µg BP | $354.00 | |||
PKC Antibody (A-9) AC | sc-17804 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKC Antibody (A-9) HRP | sc-17804 HRP | 200 µg/ml | $316.00 | |||
PKC Antibody (A-9) FITC | sc-17804 FITC | 200 µg/ml | $330.00 | |||
PKC Antibody (A-9) PE | sc-17804 PE | 200 µg/ml | $343.00 | |||
PKC Antibody (A-9) Alexa Fluor® 488 | sc-17804 AF488 | 200 µg/ml | $357.00 | |||
PKC Antibody (A-9) Alexa Fluor® 546 | sc-17804 AF546 | 200 µg/ml | $357.00 | |||
PKC Antibody (A-9) Alexa Fluor® 594 | sc-17804 AF594 | 200 µg/ml | $357.00 | |||
PKC Antibody (A-9) Alexa Fluor® 647 | sc-17804 AF647 | 200 µg/ml | $357.00 | |||
PKC Antibody (A-9) Alexa Fluor® 680 | sc-17804 AF680 | 200 µg/ml | $357.00 | |||
PKC Antibody (A-9) Alexa Fluor® 790 | sc-17804 AF790 | 200 µg/ml | $357.00 |