PP2A-B55-α Antibody (2G9): sc-81606

PP2A-B55-α Antibody (2G9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PP2A-B55-alpha protein of mouse, rat, human, and chicken origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-PP2A-B55-alpha antibody (2G9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-B55-alpha protein plays a crucial role in cellular signaling by regulating phosphorylation state of various substrates, which is essential for processes such as cell division, apoptosis, and maintaining cellular homeostasis. PP2A-B55-alpha protein is primarily located in cytoplasm and nucleus, where PP2A-B55-alpha forms a trimeric complex with a catalytic subunit and a variable subunit, allowing effective regulatory functions. PP2A-B55 interaction with specific substrates is vital for modulating signaling pathways, and dysregulation has been implicated in various diseases, including cancer. Understanding PP2A-B55-alpha localization and function is important for elucidating cellular process roles and potential therapeutic targets.

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PP2A-B55-α Antibody (2G9) References:

1. Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A.  |  Strack, S., et al. 1999. FEBS Lett. 460: 462-6. PMID: 10556517
2. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
3. Ppp2r2a Knockout Mice Reveal That Protein Phosphatase 2A Regulatory Subunit, PP2A-B55α, Is an Essential Regulator of Neuronal and Epidermal Embryonic Development.  |  Panicker, N., et al. 2020. Front Cell Dev Biol. 8: 358. PMID: 32582689
4. The PP2A regulatory subunit PPP2R2A controls NAD+ biosynthesis to regulate T cell subset differentiation in systemic autoimmunity.  |  Pan, W., et al. 2024. Cell Rep. 43: 114379. PMID: 38889006
5. Low PPP2R2A expression promotes sensitivity to CHK1 inhibition in high-grade serous ovarian cancer.  |  Qiu, Z., et al. 2024. Theranostics. 14: 7450-7469. PMID: 39659585
6. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
7. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
8. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
9. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
10. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
11. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
12. Brain protein phosphatase 2A: developmental regulation and distinct cellular and subcellular localization by B subunits.  |  Strack, S., et al. 1998. J Comp Neurol. 392: 515-27. PMID: 9514514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B55-α Antibody (2G9) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B55-α Antibody (2G9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B55-α Antibody (2G9) Specifications

Alternate Product Name: PP2A phosphatase regulatory subunit B55 alpha

Product Clone Name: 2G9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 398-411

Epitope Target: PP2A-B55α

Epitope Species: rat

Product Reactivity/Detection: PP2A-B55α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: rabbit and porcine

Additional Non-Mammalian Reactivity: chicken, frog (Xenopus laevis)

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for PP2A-B55-α Antibody (2G9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PP2A-B55 antibody is PP2A-B55 Antibody (D-10): sc-365282.