BRAF Antibody (F-7): sc-5284

Raf-B Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Raf-B of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. Raf-B Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Raf-B monoclonal antibody (F-7) plays a crucial role in the regulation of cell signaling pathways, particularly in the mitogen-activated protein kinase (MAPK) cascade, which is essential for cell growth, differentiation, and survival. Raf-B (F-7) antibody acts as a key mediator in transmitting signals from growth factors and hormones to the nucleus, influencing cellular responses to external stimuli. Raf-B is predominantly expressed in the cerebrum and testis, indicating potential involvement in specific physiological processes within these tissues. Regulation of Raf-B activity is critical, as aberrant signaling can lead to uncontrolled cell proliferation and has been implicated in various cancers. Raf-B (F-7) monoclonal antibody enables researchers to gain insights into the functional dynamics of Raf-B in different biological contexts, enhancing understanding of its role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BRAF Antibody (F-7) References:

1. The role of B-RAF in melanoma.  |  Gray-Schopfer, VC., et al. 2005. Cancer Metastasis Rev. 24: 165-83. PMID: 15785879
2. Expression of raf family proto-oncogenes in normal mouse tissues.  |  Storm, SM., et al. 1990. Oncogene. 5: 345-51. PMID: 1690378
3. Is B-Raf a good therapeutic target for melanoma and other malignancies?  |  Madhunapantula, SV. and Robertson, GP. 2008. Cancer Res. 68: 5-8. PMID: 18172288
4. Protein kinase cascades in meiotic and mitotic cell cycle control.  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
5. Mutational activation of c-raf-1 and definition of the minimal transforming sequence.  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
6. Expression of the c-raf protooncogene in human hematopoietic cells and cell lines.  |  Sariban, E., et al. 1987. Blood. 69: 1437-40. PMID: 2436688
7. Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
8. B-Raf deficiency impairs tumor initiation and progression in a murine breast cancer model.  |  Köhler, M., et al. 2019. Oncogene. 38: 1324-1339. PMID: 30659267
9. Systemic review on B-RafV600E mutation as potential therapeutic target for the treatment of cancer.  |  Chavda, J. and Bhatt, H. 2020. Eur J Med Chem. 206: 112675. PMID: 32798788
10. Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
11. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
12. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus.  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRAF Antibody (F-7) has 295 citations in a variety of scientific publications.

Primary antibodies like Anti-BRAF Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BRAF Antibody (F-7) Specifications

Alternate Product Name: BRAF

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 12-156

Epitope Target: Raf-B

Epitope Species: human

Product Reactivity/Detection: Raf-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 295 citations in various scientific publications

Available Conjugates/Forms for BRAF Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin