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p-Raf-1 Antibody (E-1): sc-271929

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Datasheets
  • p-Raf-1 Antibody (E-1) is a mouse monoclonal IgG2b κ, cited in 6 publications, provided at 200 µg/ml
  • raised against a short amino acid sequence containing phosphorylated Ser 621 of Raf-1 of human origin
  • p-Raf-1 Antibody (E-1) is recommended for detection of Ser 621 phosphorylated Raf-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine and avian
  • Anti-p-Raf-1 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Raf-1 (E-1): sc-271929.
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for p-Raf-1 Antibody (E-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-Raf-1 Antibody (E-1) (see ordering information below).

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p-Raf-1 Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 621 phosphorylated Raf-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Raf-1 (E-1) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Raf-1, also known as c-Raf, is a crucial cytoplasmic protein that plays a significant role in cell signaling pathways, particularly in regulating cell growth and differentiation. Raf-1′s function as a serine/threonine kinase is vital for activating the mitogen-activated protein kinase (MAPK) pathway, which is essential for cellular responses to growth factors and stress signals. Phosphorylation of Raf-1 at Ser 621 is necessary for catalytic activity, enabling downstream activation of MEK and ERK, which are key players in cell proliferation and survival. Raf-1′s activity is tightly regulated by various post-translational modifications, including phosphorylation by protein kinase A, which can inhibit function and prevent uncontrolled cell growth, highlighting the importance of precise regulation in maintaining cellular homeostasis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

p-Raf-1 Antibody (E-1) References:

  1. Phosphatidylinositol 3-kinase regulates Raf1 through Pak phosphorylation of serine 338.  |  Chaudhary, A., et al. 2000. Curr Biol. 10: 551-4. PMID: 10801448
  2. Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259.  |  Dhillon, AS., et al. 2002. Mol Cell Biol. 22: 3237-46. PMID: 11971957
  3. Mutational activation of c-raf-1 and definition of the minimal transforming sequence.  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
  4. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
  5. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus.  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607
  6. Negative regulation of Raf-1 by phosphorylation of serine 621.  |  Mischak, H., et al. 1996. Mol Cell Biol. 16: 5409-18. PMID: 8816453
  7. Phosphorylation of Raf-1 serine 338-serine 339 is an essential regulatory event for Ras-dependent activation and biological signaling.  |  Diaz, B., et al. 1997. Mol Cell Biol. 17: 4509-16. PMID: 9234708
  8. Oncogenes, growth factors and phorbol esters regulate Raf-1 through common mechanisms.  |  Barnard, D., et al. 1998. Oncogene. 17: 1539-47. PMID: 9794231
  9. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338.  |  King, AJ., et al. 1998. Nature. 396: 180-3. PMID: 9823899

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

p-Raf-1 Antibody (E-1)

sc-271929
200 µg/ml
$316.00

p-Raf-1 Antibody (E-1): m-IgG2b BP-HRP Bundle

sc-548710
200 µg Ab; 10 µg BP
$354.00

p-Raf-1 Antibody (E-1) AC

sc-271929 AC
500 µg/ml, 25% agarose
$416.00

p-Raf-1 Antibody (E-1) HRP

sc-271929 HRP
200 µg/ml
$316.00

p-Raf-1 Antibody (E-1) FITC

sc-271929 FITC
200 µg/ml
$330.00

p-Raf-1 Antibody (E-1) PE

sc-271929 PE
200 µg/ml
$343.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 488

sc-271929 AF488
200 µg/ml
$357.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 546

sc-271929 AF546
200 µg/ml
$357.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 594

sc-271929 AF594
200 µg/ml
$357.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 647

sc-271929 AF647
200 µg/ml
$357.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 680

sc-271929 AF680
200 µg/ml
$357.00

p-Raf-1 Antibody (E-1) Alexa Fluor® 790

sc-271929 AF790
200 µg/ml
$357.00

p-Raf-1 (E-1) Neutralizing Peptide

sc-271929 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271929 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 磷酸化raf-1磷酸化条带明显,使用的试用装,一次跑出,背景有点黑还可以再调整抗体浓度,明显,使用的试用装,一次跑出,背景有点黑还可以再调整抗体浓度,明显,使用的试用装,一次跑出,背景有点黑还可以再调整抗体浓度
Date published: 2024-07-12
Rated 5 out of 5 by from バンドの位置が正確です膵癌細胞株で、データーシートにあるように、1:100でWBを行った。かなり濃く出たので、もう少し希釈倍率を上げたほうが良いようです。
Date published: 2021-12-13
Rated 5 out of 5 by from Great blotI obtained some very clear blots from A549 and CL1-5 cell lysates
Date published: 2021-03-17
Rated 4 out of 5 by from goodThis antibody is not as good as I supposed. We used at a 1:500 dilution.
Date published: 2018-02-14
Rated 5 out of 5 by from Good antibodyGood antibody for a very fat target band but have a week band with around 100Kd at healthy human liver sample
Date published: 2017-12-04
Rated 4 out of 5 by from gut für Western BlotAntikörper gutes Signal, wenig Hintergrund Verwendung mit 1:500 Verdünnung
Date published: 2017-10-17
Rated 5 out of 5 by from It works very well.I bought this antibody two month ago. It works very well, especially in background.
Date published: 2017-08-29
Rated 3 out of 5 by from Multiple bandsAntibody produced multiple bands at 65 kDa (strongest), 80 kDa and 140 kDa in western blot using 14 ug total protein from human airway smooth muscle cells.
Date published: 2017-08-17
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p-Raf-1 Antibody (E-1) is rated 4.4 out of 5 by 15.
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