p-Raf-1 Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 621 phosphorylated Raf-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Raf-1 (E-1) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Raf-1, also known as c-Raf, is a crucial cytoplasmic protein that plays a significant role in cell signaling pathways, particularly in regulating cell growth and differentiation. Raf-1′s function as a serine/threonine kinase is vital for activating the mitogen-activated protein kinase (MAPK) pathway, which is essential for cellular responses to growth factors and stress signals. Phosphorylation of Raf-1 at Ser 621 is necessary for catalytic activity, enabling downstream activation of MEK and ERK, which are key players in cell proliferation and survival. Raf-1′s activity is tightly regulated by various post-translational modifications, including phosphorylation by protein kinase A, which can inhibit function and prevent uncontrolled cell growth, highlighting the importance of precise regulation in maintaining cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Raf-1 Antibody (E-1) | sc-271929 | 200 µg/ml | $316.00 | |||
p-Raf-1 Antibody (E-1): m-IgG2b BP-HRP Bundle | sc-548710 | 200 µg Ab; 10 µg BP | $354.00 | |||
p-Raf-1 Antibody (E-1) AC | sc-271929 AC | 500 µg/ml, 25% agarose | $416.00 | |||
p-Raf-1 Antibody (E-1) HRP | sc-271929 HRP | 200 µg/ml | $316.00 | |||
p-Raf-1 Antibody (E-1) FITC | sc-271929 FITC | 200 µg/ml | $330.00 | |||
p-Raf-1 Antibody (E-1) PE | sc-271929 PE | 200 µg/ml | $343.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 488 | sc-271929 AF488 | 200 µg/ml | $357.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 546 | sc-271929 AF546 | 200 µg/ml | $357.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 594 | sc-271929 AF594 | 200 µg/ml | $357.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 647 | sc-271929 AF647 | 200 µg/ml | $357.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 680 | sc-271929 AF680 | 200 µg/ml | $357.00 | |||
p-Raf-1 Antibody (E-1) Alexa Fluor® 790 | sc-271929 AF790 | 200 µg/ml | $357.00 | |||
p-Raf-1 (E-1) Neutralizing Peptide | sc-271929 P | 100 µg/0.5 ml | $68.00 |