TRAP Antibody (D-3): sc-376875
- TRAP Antibody (D-3) is a mouse monoclonal IgG1 κ TRAP antibody, cited in 33 publications, provided at 200 µg/ml
- raised against amino acids 24-323 mapping at the C-terminus of TRAP of human origin
- TRAP Antibody (D-3) is recommended for detection of TRAP of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TRAP Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRAP (D-3): sc-376875.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TRAP Antibody (D-3). This work is in progress.
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SEE ALSO...
TRAP Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-TRAP antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tartrate-resistant acid phosphatase (TRAP, ACP5) is an iron-containing glycoprotein that plays a crucial role in bone metabolism by catalyzing the hydrolysis of phosphate esters, which is essential for bone resorption and remodeling. TRAP is primarily located in osteoclasts, the cells responsible for bone degradation, and TRAP activity is vital for maintaining bone homeostasis. TRAP regulation is particularly important in pathological conditions such as osteoporosis and certain cancers, where osteoclast activity is dysregulated. Additionally, receptor activator of NFkB ligand (RANKL) activates TRAP, which enhances osteoclast differentiation and function, further underscoring TRAP significance in bone health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRAP Antibody (D-3) References:
- The human tartrate-resistant acid phosphatase (TRAP): involvement of the hemin responsive elements (HRE) in transcriptional regulation. | Fleckenstein, EC., et al. 2000. Leuk Lymphoma. 36: 603-12. PMID: 10784406
- Evaluation of tartrate-resistant acid phosphatase (TRAP) 5b as serum marker of bone metastases in human breast cancer. | Capeller, B., et al. 2003. Anticancer Res. 23: 1011-5. PMID: 12820340
- Blockade of the sympathetic nervous system degrades ligament in a rat MCL model. | Dwyer, KW., et al. 2004. J Appl Physiol (1985). 96: 711-8. PMID: 14527964
- Regulation of osteoclast protease expression by RANKL. | Wittrant, Y., et al. 2003. Biochem Biophys Res Commun. 310: 774-8. PMID: 14550270
- Eccentric localization of osteocytes expressing enzymatic activities, protein, and mRNA signals for type 5 tartrate-resistant acid phosphatase (TRAP). | Nakano, Y., et al. 2004. J Histochem Cytochem. 52: 1475-82. PMID: 15505342
- Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy. | Hayman, AR. 2008. Autoimmunity. 41: 218-23. PMID: 18365835
- Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells. | Reithmeier, A., et al. 2017. BMC Cancer. 17: 650. PMID: 28915803
- A Potent Tartrate Resistant Acid Phosphatase Inhibitor to Study the Function of TRAP in Alveolar Macrophages. | Boorsma, CE., et al. 2017. Sci Rep. 7: 12570. PMID: 28974738
- Identification of inhibitors of Tartrate-resistant acid phosphatase (TRAP/ACP5) activity by small-molecule screening. | Reithmeier, A., et al. 2018. Chem Biol Drug Des. 92: 1255-1271. PMID: 29500863
- Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes bone length, regulates cortical and trabecular bone mass, and maintains growth plate architecture and width in a sex- and site-specific manner in mice. | Rathod, B., et al. 2024. Bone. 188: 117223. PMID: 39111379
- Characterization and expression of tartrate-resistant acid phosphatase (TRAP) in hematopoietic cells. | Drexler, HG. and Gignac, SM. 1994. Leukemia. 8: 359-68. PMID: 8127141
- Cloning and characterization of the human tartrate-resistant acid phosphatase (TRAP) gene. | Fleckenstein, E., et al. 1996. Leukemia. 10: 637-43. PMID: 8618440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAP Antibody (D-3) | sc-376875 | 200 µg/ml | $316.00 | |||
TRAP Antibody (D-3) AC | sc-376875 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TRAP Antibody (D-3) HRP | sc-376875 HRP | 200 µg/ml | $316.00 | |||
TRAP Antibody (D-3) FITC | sc-376875 FITC | 200 µg/ml | $330.00 | |||
TRAP Antibody (D-3) PE | sc-376875 PE | 200 µg/ml | $343.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 488 | sc-376875 AF488 | 200 µg/ml | $357.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 546 | sc-376875 AF546 | 200 µg/ml | $357.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 594 | sc-376875 AF594 | 200 µg/ml | $357.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 647 | sc-376875 AF647 | 200 µg/ml | $357.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 680 | sc-376875 AF680 | 200 µg/ml | $357.00 | |||
TRAP Antibody (D-3) Alexa Fluor® 790 | sc-376875 AF790 | 200 µg/ml | $357.00 |