Target
Human GCC2
Synonyms
GCC2 | CLL-associated antigen KW-11 | CTCL tumor antigen se1-1 | GCC185 | GCC protein, 185-kD | Ran-binding protein 2-like 4 | RANBP2L4 | REN53 | KIAA0336
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QKLTNKNNKIED, from the internal region of the protein sequence according to NP_055450.1NP_852118.1.
Specificity
Human GCC2. This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 60kD band in Human Lymph Node and Testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kD according to NP_055450.1 and of 196kD according to NP_852118.1. The 60kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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