IFN-γ/Interferon gamma Antibody (G-23): sc-8423

IFN-γ Antibody (G-23) is a mouse monoclonal IgG1 kappa light chain antibody that detects Interferon gamma (IFN-γ) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IFN-γ Antibody (G-23) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Interferon gamma plays a crucial role in the immune response, acting as an antiviral and antiparasitic agent produced primarily by CD4+/CD8+ lymphocytes and natural killer cells upon activation by antigens, mitogens, or alloantigens. IFN-γ modulates T cell growth and differentiation while inhibiting B cell proliferation, thus maintaining a balanced immune response. The active form of IFN-γ is a homodimer, with each subunit comprising six helices, and this dimeric structure is stabilized by non-covalent interactions at the interface of the helices. The precursor of IFN-γ consists of 166 amino acids, which includes a 23 amino acid secretory sequence, and IFN-γ functionality can be influenced by various post-translational modifications, including glycosylation, leading to the existence of multiple forms under non-denaturing conditions, such as dimers and tetramers.

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IFN-γ/Interferon gamma Antibody (G-23) References:

1. The antiviral response to gamma interferon.  |  Costa-Pereira, AP., et al. 2002. J Virol. 76: 9060-8. PMID: 12186889
2. Histone deacetylase 1/mSin3A disrupts gamma interferon-induced CIITA function and major histocompatibility complex class II enhanceosome formation.  |  Zika, E., et al. 2003. Mol Cell Biol. 23: 3091-102. PMID: 12697811
3. Interferon-gamma: an overview of signals, mechanisms and functions.  |  Schroder, K., et al. 2004. J Leukoc Biol. 75: 163-89. PMID: 14525967
4. Interferon-gamma activation of polymorphonuclear neutrophil function.  |  Ellis, TN. and Beaman, BL. 2004. Immunology. 112: 2-12. PMID: 15096178
5. Inhibitor of kappaB kinase is required to activate a subset of interferon gamma-stimulated genes.  |  Sizemore, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 7994-8. PMID: 15148408
6. Interferon-gamma-dependent tyrosine phosphorylation of MEKK4 via Pyk2 is regulated by annexin II and SHP2 in keratinocytes.  |  Halfter, UM., et al. 2005. Biochem J. 388: 17-28. PMID: 15601262
7. Unique aspects of IFN-γ/STAT1 signaling in neurons.  |  Clark, DN., et al. 2022. Immunol Rev. 311: 187-204. PMID: 35656941
8. Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.  |  Rosain, J., et al. 2023. Cell. 186: 621-645.e33. PMID: 36736301
9. SEPTIN2 suppresses an IFN-γ-independent, proinflammatory macrophage activation pathway.  |  Fu, B., et al. 2023. Nat Commun. 14: 7441. PMID: 37978190
10. Role of interferon-gamma in immune cell regulation.  |  Young, HA. and Hardy, KJ. 1995. J Leukoc Biol. 58: 373-81. PMID: 7561512
11. Overview of interleukin-18: more than an interferon-gamma inducing factor.  |  Dinarello, CA., et al. 1998. J Leukoc Biol. 63: 658-64. PMID: 9620656
12. Regulation of interferon-gamma production by IL-12 and IL-18.  |  Okamura, H., et al. 1998. Curr Opin Immunol. 10: 259-64. PMID: 9638361

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFN-γ/Interferon gamma Antibody (G-23) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-IFN-γ/Interferon gamma Antibody (G-23) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFN-γ/Interferon gamma Antibody (G-23) Specifications

Alternate Product Name: IFN-γ

Product Clone Name: G-23

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 22-166

Epitope Target: IFN-γ

Epitope Species: human

Product Reactivity/Detection: precursor and mature IFN-γ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for IFN-γ/Interferon gamma Antibody (G-23): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin