Neuregulin-1/NRG1 Antibody (D-10): sc-393009

Neuregulin-1α/β1/2 Antibody (D-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Neuregulin-1 alpha / beta 1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Neuregulin-1α/β1/2 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neuregulin-1 protein plays a crucial role in cell signaling, particularly in the development and function of the nervous system, where Neuregulin-1 is involved in regulating cell migration, differentiation of neural crest and Schwann cells, and upregulation of acetylcholine receptors at muscle fibers during neuromuscular junction formation. This signaling is vital for proper neural development and function, and dysregulation of Neuregulin-1 has been implicated in various neurological disorders, including schizophrenia. Neuregulin-1′s diverse isoforms, generated through alternative splicing, exhibit unique structural features and functions, allowing for a wide range of biological activities and tissue-specific roles. Understanding Neuregulin-1′s interactions and functions is essential for elucidating neural development impact and potential therapeutic targets for related disorders.

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Neuregulin-1/NRG1 Antibody (D-10) References:

1. Receptor binding and biological activity of mammalian expressed sensory and motor neuron-derived factor (SMDF).  |  Osheroff, PL., et al. 1999. Growth Factors. 16: 241-53. PMID: 10372964
2. Identification of heregulin, a specific activator of p185erbB2.  |  Holmes, WE., et al. 1992. Science. 256: 1205-10. PMID: 1350381
3. Multiple novel transcription initiation sites for NRG1.  |  Steinthorsdottir, V., et al. 2004. Gene. 342: 97-105. PMID: 15527969
4. Neuregulin-1alpha and beta isoform expression in cardiac microvascular endothelial cells and function in cardiac myocytes in vitro.  |  Cote, GM., et al. 2005. Exp Cell Res. 311: 135-46. PMID: 16185687
5. Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain.  |  Bernstein, HG., et al. 2006. Brain Res Bull. 69: 546-59. PMID: 16647583
6. Meta-analysis shows strong positive association of the neuregulin 1 (NRG1) gene with schizophrenia.  |  Li, D., et al. 2006. Hum Mol Genet. 15: 1995-2002. PMID: 16687441
7. Oxygen restriction of neonate rats elevates neuregulin-1alpha isoform levels: possible relationship to schizophrenia.  |  Nadri, C., et al. 2007. Neurochem Int. 51: 447-50. PMID: 17630047
8. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.  |  Coussens, L., et al. 1985. Science. 230: 1132-9. PMID: 2999974
9. Functional EGF domain of the human neuregulin 1α produced in Escherichia coli with accurate disulfide bonds.  |  Ferreira, AS., et al. 2022. Mol Biol Rep. 49: 11715-11727. PMID: 36198848
10. Glial growth factors are alternatively spliced erbB2 ligands expressed in the nervous system.  |  Marchionni, MA., et al. 1993. Nature. 362: 312-8. PMID: 8096067
11. Isoform-specific expression and function of neuregulin.  |  Meyer, D., et al. 1997. Development. 124: 3575-86. PMID: 9342050
12. The ErbB2 and ErbB3 receptors and their ligand, neuregulin-1, are essential for development of the sympathetic nervous system.  |  Britsch, S., et al. 1998. Genes Dev. 12: 1825-36. PMID: 9637684

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neuregulin-1/NRG1 Antibody (D-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Neuregulin-1/NRG1 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neuregulin-1/NRG1 Antibody (D-10) Specifications

Alternate Product Name: Neuregulin-1α/β1/2

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 414-425

Epitope Target: Neuregulin-1 isoform HRG-α

Epitope Species: human

Product Reactivity/Detection: Neuregulin-1 isoforms HRG-α, HRG-α1A, HRG-α2B, HRG-β1, HRG-β2, and Type IV-β1a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Neuregulin-1/NRG1 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Neuregulin-1/NRG1 antibody is Neuregulin-1/NRG1 Antibody (E-12): sc-393006.