BRS-3 Antibody (B-5): sc-271712

BRS-3 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRS-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BRS-3 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Bombesin receptor subtype-3 (BRS-3) is an integral membrane protein that belongs to the G protein-coupled receptor 1 family, with its gene located on chromosome Xq26.3. BRS-3 plays a crucial role in sperm cell division, maturation, and function, with signaling mediated through G protein interactions that activate a phosphatidylinositol-calcium second messenger system. BRS-3 is expressed in germ cells within the testis and in lung carcinoma cells, but unlike other bombesin receptors, BRS-3 is not typically found in the gut and central nervous system, although BRS-3 has been detected in the rat gastrointestinal tract. Studies have shown that mice lacking BRS-3 develop obesity, indicating potential involvement in plasma insulin concentration regulation, which underscores the significance of BRS-3 in metabolic processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BRS-3 Antibody (B-5) References:

1. Bombesin receptor subtype-3 modulates plasma insulin concentration.  |  Matsumoto, K., et al. 2003. Peptides. 24: 83-90. PMID: 12576088
2. Design of selective peptidomimetic agonists for the human orphan receptor BRS-3.  |  Weber, D., et al. 2003. J Med Chem. 46: 1918-30. PMID: 12723954
3. The design of a new potent and selective ligand for the orphan bombesin receptor subtype 3 (BRS3).  |  Boyle, RG., et al. 2005. J Pept Sci. 11: 136-41. PMID: 15635635
4. Bombesin receptor subtype-3 is expressed by the enteric nervous system and by interstitial cells of Cajal in the rat gastrointestinal tract.  |  Porcher, C., et al. 2005. Cell Tissue Res. 320: 21-31. PMID: 15726424
5. Comparative pharmacology of bombesin receptor subtype-3, nonpeptide agonist MK-5046, a universal peptide agonist, and peptide antagonist Bantag-1 for human bombesin receptors.  |  Moreno, P., et al. 2013. J Pharmacol Exp Ther. 347: 100-16. PMID: 23892571
6. Bombesin receptor subtype 3 as a potential target for obesity and diabetes.  |  González, N., et al. 2015. Expert Opin Ther Targets. 19: 1153-70. PMID: 26066663
7. Novel chiral-diazepines function as specific, selective receptor agonists with variable coupling and species variability in human, mouse and rat BRS-3 receptor cells.  |  Ramos-Álvarez, I., et al. 2016. Peptides. 75: 8-17. PMID: 26524625
8. A possible new target in lung-cancer cells: The orphan receptor, bombesin receptor subtype-3.  |  Moreno, P., et al. 2018. Peptides. 101: 213-226. PMID: 29410320
9. Development and Characterization of a Novel, High-Affinity, Specific, Radiolabeled Ligand for BRS-3 Receptors.  |  Ramos-Alvarez, I., et al. 2019. J Pharmacol Exp Ther. 369: 454-465. PMID: 30971479
10. Discovery of oridonin as a novel agonist for BRS-3.  |  Zhu, Y., et al. 2022. Phytomedicine. 100: 154085. PMID: 35405616
11. Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus.  |  Gorbulev, V., et al. 1994. FEBS Lett. 340: 260-4. PMID: 8131855
12. BRS-3: a novel bombesin receptor subtype selectively expressed in testis and lung carcinoma cells.  |  Fathi, Z., et al. 1993. J Biol Chem. 268: 5979-84. PMID: 8383682

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRS-3 Antibody (B-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-BRS-3 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BRS-3 Antibody (B-5) Specifications

Alternate Product Name: BRS-3

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 27-84

Epitope Target: Bombesin Receptor Subtype-3

Epitope Species: human

Product Reactivity/Detection: BRS-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for BRS-3 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin