GULP Antibody (E-4): sc-374591
- GULP Antibody (E-4) is a mouse monoclonal IgG1 κ GULP antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of GULP of human origin
- GULP Antibody (E-4) is recommended for detection of GULP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GULP Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GULP (E-4): sc-374591.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GULP Antibody (E-4) for WB applications. These reagents are now offered in bundles with GULP Antibody (E-4) (see ordering information below).
QUICK LINKS
SEE ALSO...
GULP (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GULP protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-GULP antibody (E-4) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. GULP (engulfment adaptor PTB domain containing 1) is a 304 amino acid protein encoded by the human gene GULP1. GULP functions as an adapter protein, required for efficient phagocytosis of apoptotic cells. GULP helps modulate cellular glycosphingolipid and cholesterol transport and may play a role in internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increased cytoplasmic levels of GULP are associated with increases in cellular levels of GTP-bound ARF6. Found as a homodimer, GULP interacts with Clathrin, GDP-bound ARF6, but not with GTP-bound ARF6. GULP exists as part of a complex composed of GULP1, CENTB1 and ARF6. GULP is widely expressed and can be detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GULP Antibody (E-4) References:
- The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells. | Smits, E., et al. 1999. Curr Biol. 9: 1351-4. PMID: 10574763
- Human CED-6 encodes a functional homologue of the Caenorhabditis elegans engulfment protein CED-6. | Liu, QA. and Hengartner, MO. 1999. Curr Biol. 9: 1347-50. PMID: 10574771
- Identification and characterization of a dimerization domain in CED-6, an adapter protein involved in engulfment of apoptotic cells. | Su, HP., et al. 2000. J Biol Chem. 275: 9542-9. PMID: 10734103
- Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP). | Su, HP., et al. 2002. J Biol Chem. 277: 11772-9. PMID: 11729193
- Identification of a mouse orthologue of the CED-6 gene of Caenorhabditis elegans. | Banerjee, H., et al. 2003. Plasmid. 49: 30-3. PMID: 12583998
- The lipoprotein receptor-related protein-1 (LRP) adapter protein GULP mediates trafficking of the LRP ligand prosaposin, leading to sphingolipid and free cholesterol accumulation in late endosomes and impaired efflux. | Kiss, RS., et al. 2006. J Biol Chem. 281: 12081-92. PMID: 16497666
- Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans. | Liu, QA. and Hengartner, MO. 1998. Cell. 93: 961-72. PMID: 9635426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GULP Antibody (E-4) | sc-374591 | 200 µg/ml | $316.00 | |||
GULP Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-529777 | 200 µg Ab; 10 µg BP | $354.00 | |||
GULP Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-522786 | 200 µg Ab, 40 µg BP | $354.00 | |||
GULP Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-543684 | 200 µg Ab; 20 µg BP | $354.00 | |||
GULP Antibody (E-4) AC | sc-374591 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GULP Antibody (E-4) HRP | sc-374591 HRP | 200 µg/ml | $316.00 | |||
GULP Antibody (E-4) FITC | sc-374591 FITC | 200 µg/ml | $330.00 | |||
GULP Antibody (E-4) PE | sc-374591 PE | 200 µg/ml | $343.00 | |||
GULP Antibody (E-4) Alexa Fluor® 488 | sc-374591 AF488 | 200 µg/ml | $357.00 | |||
GULP Antibody (E-4) Alexa Fluor® 546 | sc-374591 AF546 | 200 µg/ml | $357.00 | |||
GULP Antibody (E-4) Alexa Fluor® 594 | sc-374591 AF594 | 200 µg/ml | $357.00 | |||
GULP Antibody (E-4) Alexa Fluor® 647 | sc-374591 AF647 | 200 µg/ml | $357.00 | |||
GULP Antibody (E-4) Alexa Fluor® 680 | sc-374591 AF680 | 200 µg/ml | $357.00 | |||
GULP Antibody (E-4) Alexa Fluor® 790 | sc-374591 AF790 | 200 µg/ml | $357.00 |