GM-CSFRα Antibody (E-7): sc-166955

GM-CSFRα Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects GM-CSFRα in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GM-CSFRα, or Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha, plays a crucial role in hematopoiesis and immune response by mediating the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), which is essential for the proliferation, differentiation, and survival of myeloid cells. GM-CSFRα is primarily located on the surface of hematopoietic cells, including monocytes, macrophages, and neutrophils, where GM-CSFRα facilitates the binding of GM-CSF, leading to the activation of downstream signaling pathways that promote cell survival and function. Understanding GM-CSFRα localization is vital, as GM-CSFRα expression levels can influence immune responses and the development of various hematological disorders. GM-CSFRα also interacts with the common β subunit, which is shared with the receptors for interleukin-3 and interleukin-5, highlighting GM-CSFRα importance in the regulation of multiple cytokine signaling pathways. Anti-GM-CSFRα antibody (E-7) is an invaluable tool for researchers studying the role of GM-CSF in immune regulation and its implications in diseases such as asthma, cancer, and other inflammatory conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GM-CSFRα Antibody (E-7) References:

1. Differential regulation of human eosinophil IL-3, IL-5, and GM-CSF receptor alpha-chain expression by cytokines: IL-3, IL-5, and GM-CSF down-regulate IL-5 receptor alpha expression with loss of IL-5 responsiveness, but up-regulate IL-3 receptor alpha expression.  |  Gregory, B., et al. 2003. J Immunol. 170: 5359-66. PMID: 12759409
2. Discovery and characterization of a novel splice variant of the GM-CSF receptor alpha subunit.  |  Pelley, JL., et al. 2007. Exp Hematol. 35: 1483-94. PMID: 17681666
3. Characterisation of the expression and function of the GM-CSF receptor alpha-chain in mice.  |  Rosas, M., et al. 2007. Eur J Immunol. 37: 2518-28. PMID: 17694571
4. Mavrilimumab, a human monoclonal antibody targeting GM-CSF receptor-α, in subjects with rheumatoid arthritis: a randomised, double-blind, placebo-controlled, phase I, first-in-human study.  |  Burmester, GR., et al. 2011. Ann Rheum Dis. 70: 1542-9. PMID: 21613310
5. Stage-specific changes in the levels of granulocyte-macrophage colony-stimulating factor and its receptor in the biological fluid and organ of mouse fetuses.  |  Matsumoto, A., et al. 2011. Congenit Anom (Kyoto). 51: 183-6. PMID: 22103458
6. Mavrilimumab, a human monoclonal GM-CSF receptor-α antibody for the management of rheumatoid arthritis: a novel approach to therapy.  |  Nair, JR., et al. 2012. Expert Opin Biol Ther. 12: 1661-8. PMID: 23094973
7. Blocking GM-CSF receptor α with mavrilimumab reduces infiltrating cells, pro-inflammatory markers and neoangiogenesis in ex vivo cultured arteries from patients with giant cell arteritis.  |  Corbera-Bellalta, M., et al. 2022. Ann Rheum Dis. 81: 524-536. PMID: 35045965
8. The cytoplasmic domain of granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha subunit is essential for both GM-CSF-mediated growth and differentiation.  |  Matsuguchi, T., et al. 1997. J Biol Chem. 272: 17450-9. PMID: 9211889
9. Expression and functional analysis of two isoforms of the human GM-CSF receptor alpha chain in myeloid development and leukaemia.  |  Crosier, KE., et al. 1997. Br J Haematol. 98: 540-8. PMID: 9332306
10. IL-3, IL-5, granulocyte-macrophage colony-stimulating factor receptor alpha-subunit, and common beta-subunit expression by peripheral leukocytes and blood dendritic cells.  |  Yamada, T., et al. 1998. J Allergy Clin Immunol. 101: 677-82. PMID: 9600506

The preferred GM-CSFRα antibody is GM-CSFRα Antibody (S-50): sc-456.