IFN-γRα Antibody (GIR-94): sc-12755

IFN-γRα Antibody (GIR-94) is a mouse monoclonal IgG2b kappa light chain antibody that detects the IFN-gamma R alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). IFN-γRα monoclonal antibody (GIR-94) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The IFN-gamma receptor plays a crucial role in mediating the effects of interferon-gamma, a key cytokine involved in immune responses. Upon binding of interferon-gamma, the receptor undergoes a conformational change that activates associated Janus kinases, leading to the phosphorylation of transcription factors such as Stat1. This process is vital for the transcriptional activation of genes involved in antiviral responses, immune regulation, and cell proliferation. The receptor is composed of two subunits, IFN-γRα and IFN-γRβ, with the latter being essential for signal transduction and the full biological response to interferon-gamma. Notably, while IFN-γRα is sufficient for ligand binding, the presence of both chains is necessary for the activation of IFN-γ-inducible genes, highlighting the importance of their interaction in immune signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IFN-γRα Antibody (GIR-94) References:

1. Increasing affinity of interferon-γ receptor 1 to interferon-γ by computer-aided design.  |  Mikulecký, P., et al. 2013. Biomed Res Int. 2013: 752514. PMID: 24199198
2. Association between interferon gamma receptor 1-56C/T gene polymorphism and tuberculosis susceptibility: a meta-analysis.  |  Wang, W., et al. 2014. Chin Med J (Engl). 127: 3782-8. PMID: 25382336
3. The human interferon-gamma receptor. Purification, characterization, and preparation of antibodies.  |  Novick, D., et al. 1987. J Biol Chem. 262: 8483-7. PMID: 2954953
4. Molecular cloning and expression of the human interferon-gamma receptor.  |  Aguet, M., et al. 1988. Cell. 55: 273-80. PMID: 2971451
5. Interferon-γ receptor-1 gene promoter polymorphisms and susceptibility for brucellosis in Makkah region.  |  Ismael, AB., et al. 2018. Afr Health Sci. 18: 1157-1165. PMID: 30766581
6. Type I and Type II interferon receptors.  |  Orchansky, P., et al. 1984. J Interferon Res. 4: 275-82. PMID: 6086780
7. Interferon-induced nuclear signalling by Jak protein tyrosine kinases.  |  Silvennoinen, O., et al. 1993. Nature. 366: 583-5. PMID: 7504785
8. Recent progress in the elucidation of interferon-gamma actions: molecular biology and biological functions.  |  Vilcek, J. and Oliveira, IC. 1994. Int Arch Allergy Immunol. 104: 311-6. PMID: 8038608
9. Identification and sequence of an accessory factor required for activation of the human interferon gamma receptor.  |  Soh, J., et al. 1994. Cell. 76: 793-802. PMID: 8124716
10. A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.  |  Hemmi, S., et al. 1994. Cell. 76: 803-10. PMID: 8124717
11. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455
12. The molecular cell biology of interferon-gamma and its receptor.  |  Farrar, MA. and Schreiber, RD. 1993. Annu Rev Immunol. 11: 571-611. PMID: 8476573

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFN-γRα Antibody (GIR-94) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-IFN-γRα Antibody (GIR-94) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFN-γRα Antibody (GIR-94) Specifications

Alternate Product Name: CD119

Product Clone Name: GIR-94

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: IFN-γRα

Epitope Species: human

Product Reactivity/Detection: IFN-γRα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for IFN-γRα Antibody (GIR-94): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin