IL-3/IL-5/GM-CSFRβ Antibody (1C1): sc-21765

IL-3/IL-5/GM-CSFRβ Antibody (1C1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the IL-3/IL-5/GM-CSFR beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). IL-3/IL-5/GM-CSFRβ (1C1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IL-3/IL-5/GM-CSFR beta protein plays a crucial role in hematopoiesis, as part of a receptor complex that mediates the effects of interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor. This receptor complex is essential for the proliferation and differentiation of hematopoietic progenitor cells, and proper functioning maintains immune system homeostasis. The β subunit of IL-3/IL-5/GM-CSFR is shared among these cytokines and activates downstream signaling pathways, including the MAP kinase pathway, which is critical for cell survival and proliferation. Dysregulation of IL-3/IL-5/GM-CSFR beta makes anti-IL-3/IL-5/GM-CSFRβ antibody (1C1) an invaluable tool for research into hematological disorders and understanding complex signaling mechanisms in immune responses.

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IL-3/IL-5/GM-CSFRβ Antibody (1C1) References:

1. Critical cytoplasmic domains of the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors for growth signal transduction and tyrosine phosphorylation.  |  Sakamaki, K., et al. 1992. EMBO J. 11: 3541-9. PMID: 1396555
2. GM-CSF, IL-3 and IL-5: cross-competition on human haemopoietic cells.  |  Lopez, AF., et al. 1992. Immunol Today. 13: 495-500. PMID: 1463582
3. Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.  |  Park, LS., et al. 1992. Proc Natl Acad Sci U S A. 89: 4295-9. PMID: 1533931
4. Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3).  |  Hara, T. and Miyajima, A. 1992. EMBO J. 11: 1875-84. PMID: 1582416
5. Cytokine receptors and signal transduction.  |  Miyajima, A., et al. 1992. Annu Rev Immunol. 10: 295-331. PMID: 1590989
6. Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor.  |  Hayashida, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9655-9. PMID: 1702217
7. GM-CSF, IL-3, and IL-5 Family of Cytokines: Regulators of Inflammation.  |  Dougan, M., et al. 2019. Immunity. 50: 796-811. PMID: 30995500
8. The roles of IL-5 and anti-IL-5 treatment in eosinophilic diseases: Asthma, eosinophilic granulomatosis with polyangiitis, and eosinophilic chronic rhinosinusitis.  |  Nagase, H., et al. 2020. Allergol Int. 69: 178-186. PMID: 32139163
9. Human IL-3 receptor signaling: rapid induction of phosphatidylcholine hydrolysis is independent of protein kinase C but dependent on tyrosine phosphorylation in transfected NIH 3T3 cells.  |  Rao, P., et al. 1995. J Immunol. 154: 1664-74. PMID: 7836750
10. A model for the interaction of the GM-CSF, IL-3 and IL-5 receptors with their ligands.  |  Goodall, GJ., et al. 1993. Growth Factors. 8: 87-97. PMID: 8466757
11. A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF.  |  Tavernier, J., et al. 1991. Cell. 66: 1175-84. PMID: 1833065

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-3/IL-5/GM-CSFRβ Antibody (1C1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-3/IL-5/GM-CSFRβ Antibody (1C1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-3/IL-5/GM-CSFRβ Antibody (1C1) Specifications

Alternate Product Name: CD131

Product Clone Name: 1C1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: IL-3/IL-5/GM-CSFRβ

Epitope Species: human

Product Reactivity/Detection: 130 kDa β chain common to IL-3R, IL-5R and GM-CSFR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for IL-3/IL-5/GM-CSFRβ Antibody (1C1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin