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p-INSR/Insulin Receptor β Antibody (21G12): sc-81501

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Datasheets
  • p-INSR/Insulin Receptor β Antibody (21G12) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/0.5 ml
  • raised against phosphopeptide corresponding to amino acid residues surrounding tyrosine 1322 of insulin R of human origin
  • recommended for detection of Tyr 1322 phosphorylated insulin Rβ of mouse, rat, human and canine origin by WB and IP
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-INSR/Insulin Receptor β Antibody (21G12). This work is in progress.

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p-insulin Rβ Antibody (21G12) is a mouse monoclonal IgG1 antibody that detects insulin receptor beta (insulin Rβ) in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP) applications. Anti-insulin Rβ antibody (21G12) specifically recognizes the phosphorylated form of the insulin receptor beta subunit, which plays a crucial role in mediating insulin signaling effects. The insulin receptor is a heterodimeric protein complex composed of an extracellular alpha subunit and a transmembrane beta subunit, which is essential for glucose homeostasis regulation. Upon insulin binding, the receptor undergoes a conformational change that activates intrinsic tyrosine kinase activity, leading to tyrosine residue phosphorylation within the beta subunit and recruitment of various signaling proteins. This phosphorylation cascade is vital for insulin′s downstream effects, including glucose uptake and glycogen synthesis, making insulin receptor a key player in metabolic regulation. Additionally, insulin receptor undergoes post-translational modifications, such as phosphorylation at specific residues like Tyr 1322, which can influence activity and interaction with other signaling molecules. Anti-insulin Rβ antibody (21G12) detection of these modifications makes this reagent an invaluable tool for researchers studying insulin signaling pathways and their implications in metabolic disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

p-INSR/Insulin Receptor β Antibody (21G12)

sc-81501
50 µg/0.5 ml
$316.00

I am using p-insulin Rβ (21G12): sc-81501 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great resultClear background and nice image concentrate band sample can be multiple funtion test
Date published: 2021-04-09
Rated 5 out of 5 by from strong signalThis antibody gave a strong signal with no background (5% milk blocking) when used in western blot detection on MCF-7 cell.It's very good quality.
Date published: 2017-08-19
Rated 4 out of 5 by from Positive results obtained in WB anaylsis with MDAPositive results obtained in WB anaylsis with MDA-MB-231 whole cell lysates. -SCBT QC
Date published: 2013-03-08
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p-INSR/Insulin Receptor β Antibody (21G12) is rated 4.7 out of 5 by 3.
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