4.1N Antibody (B-2): sc-374367

4.1N Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects 4.1N protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-4.1N antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 4.1N protein, also known as band 4.1-like protein 1 or neuronal protein 4.1, plays a crucial role in binding and stabilizing D2 and D3 dopamine receptors at the neuronal plasma membrane, which is essential for maintaining proper neurotransmission and neuronal signaling. 4.1N protein is predominantly expressed in the brain, with lower levels found in the heart, kidney, pancreas, placenta, lung, and skeletal muscle, highlighting its significance in neural function. Additionally, 4.1N protein interacts with the cytoskeleton and the overlying plasma membrane, contributing to the stability and plasticity of neuronal membranes. The presence of four homologous genes (4.1R, 4.1G, 4.1N, and 4.1B) that undergo complex alternative splicing suggests a diverse range of functions and regulatory mechanisms, particularly in the targeting of transmembrane proteins in kidney epithelium, which may influence specific kidney functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

4.1N Antibody (B-2) References:

1. Protein 4.1N binding to nuclear mitotic apparatus protein in PC12 cells mediates the antiproliferative actions of nerve growth factor.  |  Ye, K., et al. 1999. J Neurosci. 19: 10747-56. PMID: 10594058
2. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N.  |  Ye, K., et al. 2000. Cell. 103: 919-30. PMID: 11136977
3. Functional characterization of spectrin-actin-binding domains in 4.1 family of proteins.  |  Gimm, JA., et al. 2002. Biochemistry. 41: 7275-82. PMID: 12044158
4. D2 and D3 dopamine receptor cell surface localization mediated by interaction with protein 4.1N.  |  Binda, AV., et al. 2002. Mol Pharmacol. 62: 507-13. PMID: 12181426
5. Protein 4.1N is required for translocation of inositol 1,4,5-trisphosphate receptor type 1 to the basolateral membrane domain in polarized Madin-Darby canine kidney cells.  |  Zhang, S., et al. 2003. J Biol Chem. 278: 4048-56. PMID: 12444087
6. Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 is regulated by actin filaments and 4.1N in neuronal dendrites.  |  Fukatsu, K., et al. 2004. J Biol Chem. 279: 48976-82. PMID: 15364918

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-4.1N Antibody (B-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-4.1N Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

4.1N Antibody (B-2) Specifications

Alternate Product Name: 4.1N

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 573-765

Epitope Target: 4.1N

Epitope Species: human

Product Reactivity/Detection: 4.1N

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for 4.1N Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin