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4.1R Antibody (D-11): sc-514096

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Datasheets
  • 4.1R Antibody (D-11) is a mouse monoclonal IgA κ provided at 200 µg/ml
  • raised against amino acids 81-180 mapping within an internal region of 4.1R of human origin
  • recommended for detection of 4.1R of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for 4.1R Antibody (D-11). This work is in progress.

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    4.1R Antibody (D-11) is a mouse monoclonal IgA antibody that detects 4.1R in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). 4.1R is a crucial multifunctional protein that plays a significant role in maintaining the shape and mechanical properties of erythrocyte membranes, which is vital for proper red blood cell function and overall circulatory health. 4.1R is part of the 4.1 gene family, which encodes various cytoskeletal proteins predominantly expressed in the nervous system, including 4.1G, 4.1N, and 4.1B. The unique distribution of 4.1R and 4.1G in the cerebellum suggests their involvement in modulating the mechanical properties of neuronal cell membranes by facilitating the association of fodrin and actin, thereby influencing cellular stability and signaling. Additionally, 4.1R has been associated with hereditary elliptocytosis, highlighting its importance in maintaining erythrocyte integrity. Anti-4.1R antibody (D-11) is particularly valuable for researchers studying red blood cell morphology and related disorders, as well as for those investigating the broader implications of cytoskeletal dynamics in neuronal function.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    4.1R Antibody (D-11) References:

    1. Regulation of protein 4.1R interactions with membrane proteins by Ca2+ and calmodulin.  |  Nunomura, W. and Takakuwa, Y. 2006. Front Biosci. 11: 1522-39. PMID: 16368534
    2. Cytoskeletal protein 4.1R negatively regulates T-cell activation by inhibiting the phosphorylation of LAT.  |  Kang, Q., et al. 2009. Blood. 113: 6128-37. PMID: 19190245
    3. Structural protein 4.1R is integrally involved in nuclear envelope protein localization, centrosome-nucleus association and transcriptional signaling.  |  Meyer, AJ., et al. 2011. J Cell Sci. 124: 1433-44. PMID: 21486941
    4. Phosphorylation-dependent perturbations of the 4.1R-associated multiprotein complex of the erythrocyte membrane.  |  Gauthier, E., et al. 2011. Biochemistry. 50: 4561-7. PMID: 21542582
    5. Characterization of cytoskeletal protein 4.1R interaction with NHE1 (Na(+)/H(+) exchanger isoform 1).  |  Nunomura, W., et al. 2012. Biochem J. 446: 427-35. PMID: 22731252
    6. ICln: a new regulator of non-erythroid 4.1R localisation and function.  |  Bazzini, C., et al. 2014. PLoS One. 9: e108826. PMID: 25295618
    7. The human Kell blood group binds the erythroid 4.1R protein: new insights into the 4.1R-dependent red cell membrane complex.  |  Azouzi, S., et al. 2015. Br J Haematol. 171: 862-71. PMID: 26455906
    8. Cytoskeleton protein 4.1R suppresses murine keratinocyte cell hyperproliferation via activating the Akt/ERK pathway in an EGFR-dependent manner.  |  Chen, L., et al. 2019. Exp Cell Res. 384: 111648. PMID: 31562860
    9. CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane.  |  Funaki, T., et al. 2021. Biochem Biophys Res Commun. 534: 172-178. PMID: 33298314
    10. Membrane skeleton hyperstability due to a novel alternatively spliced 4.1R can account for ellipsoidal camelid red cells with decreased deformability.  |  Chen, Y., et al. 2023. J Biol Chem. 299: 102877. PMID: 36621628

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    4.1R Antibody (D-11)

    sc-514096
    200 µg/ml
    $316.00

    I am using 4.1R (D-11): sc-514096 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: Randy McDonald
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Great Western blot data of 4Great Western blot data of 4.1R expression in non-transfected 293T, human 4.1R transfected 293T and MOLT-4 whole cell lysates. -SCBT QC
    Date published: 2013-04-26
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    4.1R Antibody (D-11) is rated 5.0 out of 5 by 1.
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