ACBP Antibody (C-9): sc-376853

ACBP Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACBP of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ACBP antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Acyl-CoA binding protein (ACBP) plays a crucial role in lipid metabolism by binding long chain acyl-CoA esters (LCAs) with high affinity, thereby regulating their availability and influencing various intracellular functions. ACBP is predominantly located in the cytoplasm, where ACBP facilitates the transport and storage of acyl-CoA, which is essential for fatty acid metabolism and energy production. ACBP is highly conserved across multiple species, including humans, rats, frogs, insects, plants, and yeast, highlighting ACBP′s fundamental biological importance. The gene encoding human ACBP, DBI, is located on chromosome 2 and is highly expressed in tissues such as the liver, soleus muscle, and heart, particularly in cells engaged in lipogenesis and de novo fatty acid synthesis. Notably, ACBP expression is significantly induced during adipocyte differentiation, and ACBP is a target gene for proliferator-activated receptor (PPAR) γ, being directly activated by PPARγ/RXRα and PPARα/RXRα, but not by PPARδ/RXRα. Beyond ACBP′s role in acyl-CoA binding and transport, ACBP is also implicated in the binding of γ-aminobutyric acid type A receptors, steroidogenesis, and the release of peptide hormones, making ACBP a vital component in various metabolic pathways.

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ACBP Antibody (C-9) References:

1. Role of acyl-CoA binding protein in acyl-CoA metabolism and acyl-CoA-mediated cell signaling.  |  Knudsen, J., et al. 2000. J Nutr. 130: 294S-298S. PMID: 10721891
2. Acyl-coenzyme A binding protein expression is fibre-type specific in rat skeletal muscle but not affected by moderate endurance training.  |  Franch, J., et al. 2002. Pflugers Arch. 443: 387-93. PMID: 11810207
3. The gene encoding the Acyl-CoA-binding protein is activated by peroxisome proliferator-activated receptor gamma through an intronic response element functionally conserved between humans and rodents.  |  Helledie, T., et al. 2002. J Biol Chem. 277: 26821-30. PMID: 12015306
4. Acyl-CoA-binding protein (ACBP) and its relation to fatty acid-binding protein (FABP): an overview.  |  Knudsen, J. Mol Cell Biochem. 98: 217-23. PMID: 2266962
5. Acyl CoA binding protein (ACBP): an autophagy checkpoint that can be targeted for improving cancer immunosurveillance.  |  Montégut, L., et al. 2024. Oncoimmunology. 13: 2413200. PMID: 39381588
6. Neutralization of the autophagy-repressive tissue hormone DBI/ACBP (diazepam binding inhibitor, acyl-CoA binding protein) enhances anticancer immunosurveillance.  |  Montégut, L., et al. 2024. Autophagy. 20: 2836-2838. PMID: 39419485
7. ACBP/DBI neutralization for the experimental treatment of fatty liver disease.  |  Motiño, O., et al. 2024. Cell Death Differ.. PMID: 39550516
8. Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.  |  Pan, H., et al. 2024. Nat Metab. 6: 2281-2299. PMID: 39578649
9. Autophagy-dependent hepatocyte secretion of DBI/ACBP induced by glucocorticoids determines the pathogenesis of cushing syndrome.  |  Pan, H., et al. 2024. Autophagy.. PMID: 39663572
10. Molecular cloning and chromosomal localization of a pseudogene related to the human acyl-CoA binding protein/diazepam binding inhibitor.  |  Gersuk, VH., et al. 1995. Genomics. 25: 469-76. PMID: 7789980
11. The function of acyl-CoA-binding protein (ACBP)/diazepam binding inhibitor (DBI).  |  Knudsen, J., et al. Mol Cell Biochem. 123: 129-38. PMID: 8232254
12. Identification of diazepam-binding Inhibitor/Acyl-CoA-binding protein as a sterol regulatory element-binding protein-responsive gene.  |  Swinnen, JV., et al. 1998. J Biol Chem. 273: 19938-44. PMID: 9685328

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACBP Antibody (C-9) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ACBP Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACBP Antibody (C-9) Specifications

Alternate Product Name: ACBP

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-87

Epitope Target: ACBP

Epitope Species: human

Product Reactivity/Detection: ACBP short and long isoforms

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ACBP Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin