DnaJB6 Antibody (B-5): sc-365574

DnaJB6 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DnaJB6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DnaJB6 antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DnaJB6, also known as DnaJ homolog subfamily B member 6, plays a crucial role in cellular stress responses by functioning as a molecular chaperone. DnaJB6 is primarily located in the cytoplasm and is essential for maintaining protein homeostasis, particularly under conditions of heat shock or other stressors. DnaJB6′s ability to prevent the aggregation of misfolded proteins and assist in their proper folding is vital for cellular function and survival. Dysregulation of DnaJB6 has been implicated in various neurodegenerative diseases, highlighting DnaJB6′s importance in protecting neuronal cells from stress-induced damage. Highly expressed in the brain, DnaJB6 is also present at lower levels in other tissues, underscoring DnaJB6′s specialized role in neuronal health and function.

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DnaJB6 Antibody (B-5) References:

1. Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling.  |  Stewart, GR., et al. 2004. Tuberculosis (Edinb). 84: 180-7. PMID: 15207487
2. The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: a small angle X-ray scattering study in solution.  |  Shi, YY., et al. 2005. J Biol Chem. 280: 22761-8. PMID: 15849180
3. The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones.  |  Qiu, XB., et al. 2006. Cell Mol Life Sci. 63: 2560-70. PMID: 16952052
4. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions.  |  Genevaux, P., et al. 2007. Mol Microbiol. 66: 840-57. PMID: 17919282
5. DnaJ recruits DnaK to protein aggregates.  |  Acebrón, SP., et al. 2008. J Biol Chem. 283: 1381-1390. PMID: 17984091
6. Human DnaJB6 Antiamyloid Chaperone Protects Yeast from Polyglutamine Toxicity Separately from Spatial Segregation of Aggregates.  |  Kumar, J., et al. 2018. Mol Cell Biol. 38: PMID: 30224519
7. DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis.  |  Rosas-Salvans, M., et al. 2019. J Cell Sci. 132: PMID: 31064815
8. Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12.  |  Saito, H. and Uchida, H. 1978. Mol Gen Genet. 164: 1-8. PMID: 360036
9. The roles of HSP40/DNAJ protein family in neurodegenerative diseases.  |  He, Y. and Wang, Z. 2022. Zhejiang Da Xue Xue Bao Yi Xue Ban. 51: 640-646. PMID: 36581576
10. Identification of the E. coli dnaJ gene product.  |  Georgopoulos, CP., et al. 1980. Mol Gen Genet. 178: 583-8. PMID: 6446654
11. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli.  |  Tomoyasu, T., et al. 1998. Mol Microbiol. 30: 567-81. PMID: 9822822
12. Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ.  |  Suh, WC., et al. 1998. Proc Natl Acad Sci U S A. 95: 15223-8. PMID: 9860950

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DnaJB6 Antibody (B-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DnaJB6 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DnaJB6 Antibody (B-5) Specifications

Alternate Product Name: DnaJB6

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 110-135

Epitope Target: DnaJB6

Epitope Species: human

Product Reactivity/Detection: DnaJB6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DnaJB6 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin