γ-GCSc Antibody (H-5): sc-390811

γ-GCSc Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects gamma-GCSc protein, also known as glutamate-cysteine ligase catalytic subunit (GCLC), in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). γ-GCSc monoclonal antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GCLC gene, comprising 16 exons, encodes the 636 amino acid gamma-GCSc protein, which plays a crucial role in glutathione synthesis, a vital antioxidant that protects cells from oxidative stress. Gamma-GCSc is primarily expressed in hemocytes, brain, liver, and kidney, where gamma-GCSc forms a heterodimer with the regulatory subunit gamma-GCSm, collectively known as gamma-GCS. Gamma-GCSc catalyzes the first and rate-limiting step in glutathione biosynthesis, making gamma-GCSc essential for cellular defense against reactive oxygen species and maintaining redox homeostasis. Gamma-GCSc regulation is influenced by various factors, including oxidants and heavy metals, which can enhance transcription, thereby increasing glutathione levels and potentially providing protective effects against chemically induced carcinogenesis. Notably, mutations such as the His370Leu change in gamma-GCSc can lead to significant deficiencies in enzyme activity, resulting in conditions like hemolytic anemia and reduced glutathione levels in red blood cells.

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γ-GCSc Antibody (H-5) References:

1. The molecular basis of a case of gamma-glutamylcysteine synthetase deficiency.  |  Beutler, E., et al. 1999. Blood. 94: 2890-4. PMID: 10515893
2. Induction of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) in the brains of adult female mice subchronically exposed to methylmercury.  |  Thompson, SA., et al. 1999. Toxicol Lett. 110: 1-9. PMID: 10593589
3. Multi-faceted regulation of gamma-glutamylcysteine synthetase.  |  Soltaninassab, SR., et al. 2000. J Cell Physiol. 182: 163-70. PMID: 10623879
4. Structure of the human glutamate-L-cysteine ligase catalytic (GLCLC) subunit gene.  |  Gipp, JJ. and Mulcahy, RT. 2000. Cytogenet Cell Genet. 88: 130-2. PMID: 10773686
5. Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network.  |  Dormer, UH., et al. 2000. J Biol Chem. 275: 32611-6. PMID: 10921921
6. Genetic analysis of CC16, OGG1 and GCLC polymorphisms and susceptibility to COPD.  |  Liu, S., et al. 2007. Respirology. 12: 29-33. PMID: 17207022
7. Ferroptosis-Related Gene GCLC Is a Novel Prognostic Molecular and Correlates with Immune Infiltrates in Lung Adenocarcinoma.  |  Luo, L., et al. 2022. Cells. 11: PMID: 36359768
8. Andrographolide attenuates SARS-CoV-2 infection via an up-regulation of glutamate-cysteine ligase catalytic subunit (GCLC).  |  Chaopreecha, J., et al. 2025. Phytomedicine. 136: 156279. PMID: 39631298
9. Transport accounts for glutathione turnover in human erythrocytes.  |  Lunn, G., et al. 1979. Blood. 54: 238-44. PMID: 444668
10. Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.  |  Sierra-Rivera, E., et al. 1995. Cytogenet Cell Genet. 70: 278-9. PMID: 7789189
11. Genetic mapping of GLCLC, the human gene encoding the catalytic subunit of gamma-glutamyl-cysteine synthetase, to chromosome band 6p12 and characterization of a polymorphic trinucleotide repeat within its 5' untranslated region.  |  Walsh, AC., et al. 1996. Cytogenet Cell Genet. 75: 14-6. PMID: 8995480
12. Amino acid-dependent regulation of the Saccharomyces cerevisiae GSH1 gene by hydrogen peroxide.  |  Stephen, DW. and Jamieson, DJ. 1997. Mol Microbiol. 23: 203-10. PMID: 9044254

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ-GCSc Antibody (H-5) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-γ-GCSc Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ-GCSc Antibody (H-5) Specifications

Alternate Product Name: GCLC (glutamate cysteine ligase catalytic)

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 338-637

Epitope Target: γ-GCSc

Epitope Species: human

Product Reactivity/Detection: γ-GCSc

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 39 citations in various scientific publications

Available Conjugates/Forms for γ-GCSc Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin