GRB2 Antibody (C-7): sc-8034

GRB2 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the GRB2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GRB2 monoclonal antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GRB2 protein plays a crucial role in cellular signaling pathways, particularly in the regulation of the Ras signaling cascade, which is essential for cell growth, differentiation, and survival. By acting as an adaptor protein, GRB2 facilitates the interaction between growth factor receptors and guanine nucleotide exchange factors, such as SOS1, thereby promoting the activation of Ras in response to extracellular signals. This interaction is vital for the proper transmission of signals from the cell surface to the nucleus, influencing various cellular processes. Additionally, GRB2 is necessary during embryogenesis for the differentiation of endodermal cells and the formation of the epiblast, highlighting its importance in developmental biology. GRB2 monoclonal antibody (C-7) effectively detects and characterizes the GRB2 protein, making GRB2 antibody (C-7) an invaluable tool for researchers studying signaling pathways and their implications in health and disease.

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GRB2 Antibody (C-7) References:

1. Grb2 SH2 domain-binding peptide analogs as potential anticancer agents.  |  Lung, FD. and Tsai, JY. 2003. Biopolymers. 71: 132-40. PMID: 12767115
2. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
3. GRB2 is a BECN1 interacting protein that regulates autophagy.  |  Montero-Vergara, J., et al. 2024. Cell Death Dis. 15: 14. PMID: 38182563
4. GRB2 stabilizes RAD51 at reversed replication forks suppressing genomic instability and innate immunity against cancer.  |  Ye, Z., et al. 2024. Nat Commun. 15: 2132. PMID: 38459011
5. The Configuration of GRB2 in Protein Interaction and Signal Transduction.  |  Wang, D., et al. 2024. Biomolecules. 14: PMID: 38540680
6. The role of GRB2 in diabetes, diabetes complications and related disorders.  |  Ma, J., et al. 2025. Diabetes Obes Metab. 27: 23-34. PMID: 39478285
7. The function of GRB2 in linking the insulin receptor to Ras signaling pathways.  |  Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
8. Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1.  |  Zhang, XF., et al. 1993. Nature. 364: 308-13. PMID: 8332187
9. An SH3-SH2-SH3 protein is required for p21Ras1 activation and binds to sevenless and Sos proteins in vitro.  |  Simon, MA., et al. 1993. Cell. 73: 169-77. PMID: 8462097
10. Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation.  |  Egan, SE., et al. 1993. Nature. 363: 45-51. PMID: 8479536
11. Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor.  |  Buday, L. and Downward, J. 1993. Cell. 73: 611-20. PMID: 8490966
12. Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2.  |  Chardin, P., et al. 1993. Science. 260: 1338-43. PMID: 8493579

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRB2 Antibody (C-7) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-GRB2 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRB2 Antibody (C-7) Specifications

Alternate Product Name: GRB2

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 54-164

Epitope Target: GRB2

Epitope Species: mouse

Product Reactivity/Detection: GRB2 p25

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for GRB2 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin