GSS Antibody (C-5): sc-365863

GSS Antibody (C-5) is a mouse monoclonal IgG2b antibody that detects Glutathione synthetase (GSS) in mouse, rat, human, and hamster samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GSS is a crucial enzyme involved in the biosynthesis of glutathione, a vital antioxidant that protects cells from oxidative stress and maintains cellular redox balance. GSS (C-5) antibody targets proteins primarily located in the cytoplasm of cells, where GSS plays a significant role in detoxifying harmful compounds and regulating cellular metabolism. Proper functioning of GSS is essential for maintaining adequate levels of glutathione, and deficiencies in this enzyme can lead to serious health issues, including 5-oxoprolinuria, which is characterized by neurological damage and metabolic disorders. GSS occurs as a homodimer and is encoded by a gene located on human chromosome 20q11.2, with its structure comprising three loops projecting from an antiparallel β-sheet, a parallel β-sheet, and a lid of anti-parallel sheets that facilitate access to the ATP-binding site. Anti-GSS antibody (C-5) is an invaluable tool for researchers studying the role of glutathione in cellular processes and the implications of GSS deficiencies in various diseases.

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GSS Antibody (C-5) References:

1. Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses.  |  Li, H., et al. 2003. Proc Natl Acad Sci U S A. 100: 9785-90. PMID: 12909715
2. Function of conserved residues of human glutathione synthetase: implications for the ATP-grasp enzymes.  |  Dinescu, A., et al. 2004. J Biol Chem. 279: 22412-21. PMID: 14990577
3. S-Acetylglutathione normalizes intracellular glutathione content in cultured fibroblasts from patients with glutathione synthetase deficiency.  |  Okun, JG., et al. 2004. J Inherit Metab Dis. 27: 783-6. PMID: 15617191
4. Glutathione is essential for early embryogenesis--analysis of a glutathione synthetase knockout mouse.  |  Winkler, A., et al. 2011. Biochem Biophys Res Commun. 412: 121-6. PMID: 21802407
5. Protein levels of clusterin and glutathione synthetase in platelets allow for early detection of colorectal cancer.  |  Strohkamp, S., et al. 2018. Cell Mol Life Sci. 75: 323-334. PMID: 28849249
6. Erythrocyte glutathione synthetase deficiency leads not only to glutathione but also to glutathione-S-transferase deficiency.  |  Beutler, E., et al. 1986. J Clin Invest. 77: 38-41. PMID: 3944259
7. Glutathione synthetase deficiency, an inborn error of metabolism involving the gamma-glutamyl cycle in patients with 5-oxoprolinuria (pyroglutamic aciduria).  |  Wellner, VP., et al. 1974. Proc Natl Acad Sci U S A. 71: 2505-9. PMID: 4152248
8. Oxidative damage to neutrophils in glutathione synthetase deficiency.  |  Spielberg, SP., et al. 1979. Br J Haematol. 42: 215-23. PMID: 465367
9. Sequencing and expression of a cDNA for human glutathione synthetase.  |  Gali, RR. and Board, PG. 1995. Biochem J. 310 (Pt 1): 353-8. PMID: 7646467
10. Effect of decreased glutathione levels in hereditary glutathione synthetase deficiency on dibromoethane-induced genotoxicity in human fibroblasts.  |  DeLeve, LD. 1997. Mutat Res. 389: 291-7. PMID: 9093395

The preferred GSS antibody is GSS Antibody (H-7): sc-166882.