HS6ST1 Antibody (C-9): sc-398231

HS6ST1 Antibody (C-9) is a mouse monoclonal IgM antibody that detects HS6ST1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HS6ST1 antibody (C-9) is available as a non-conjugated format. HS6ST1, also known as heparan sulfate 6-O-sulfotransferase 1, is a crucial enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that plays a significant role in various biological processes, including cell signaling, development, and tissue repair. This 411 amino acid single-pass type II membrane protein is expressed in the fetal brain and functions specifically to catalyze the transfer of sulfate from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to the N-sulfoglucosamine residue of heparan sulfate, thereby influencing the structural diversity and biological activity of heparan sulfate chains. HS6ST1 regulates the sulfation patterns of heparan sulfate, which can affect interactions with growth factors, morphogens, and extracellular matrix components, ultimately impacting cellular behavior and tissue homeostasis. Additionally, HS6ST1 undergoes post-translational N-glycosylation, which can further modulate activity and stability. Encoded by a functional gene on human chromosome 2 and a pseudogene on chromosome 1 (LOC728969), HS6ST1 is a vital component in the intricate network of cellular signaling and development.

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HS6ST1 Antibody (C-9) References:

1. The occurrence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the targeted N-sulfoglucosamine.  |  Habuchi, H., et al. 2000. J Biol Chem. 275: 2859-68. PMID: 10644753
2. Biosynthesis of heparan sulphate with diverse structures and functions: two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties.  |  Habuchi, H., et al. 2003. Biochem J. 371: 131-42. PMID: 12492399
3. A conformational change in heparan sulfate 3-O-sulfotransferase-1 is induced by binding to heparan sulfate.  |  Edavettal, SC., et al. 2004. Biochemistry. 43: 4680-8. PMID: 15096036
4. Regulation of heparan sulfate 6-O-sulfation by beta-secretase activity.  |  Nagai, N., et al. 2007. J Biol Chem. 282: 14942-51. PMID: 17363373
5. Mice deficient in heparan sulfate 6-O-sulfotransferase-1 exhibit defective heparan sulfate biosynthesis, abnormal placentation, and late embryonic lethality.  |  Habuchi, H., et al. 2007. J Biol Chem. 282: 15578-88. PMID: 17405882
6. Heparan sulphate synthetic and editing enzymes in ovarian cancer.  |  Backen, AC., et al. 2007. Br J Cancer. 96: 1544-8. PMID: 17437011
7. Cartilage tumour progression is characterized by an increased expression of heparan sulphate 6O-sulphation-modifying enzymes.  |  Waaijer, CJ., et al. 2012. Virchows Arch. 461: 475-81. PMID: 22903264
8. Heparan Sulfate Biosynthetic System Is Inhibited in Human Glioma Due to EXT1/2 and HS6ST1/2 Down-Regulation.  |  Ushakov, VS., et al. 2017. Int J Mol Sci. 18: PMID: 29104277
9. HS6ST1 overexpressed in cancer-associated fibroblast and inhibited cholangiocarcinoma progression.  |  Hu, S., et al. 2023. Dig Liver Dis. 55: 1114-1125. PMID: 36586771
10. Genetic Architecture of Ischaemic Strokes after COVID-19 Shows Similarities with Large Vessel Strokes.  |  Llucià-Carol, L., et al. 2023. Int J Mol Sci. 24: PMID: 37686257
11. B3GALT6 promotes dormant breast cancer cell survival and recurrence by enabling heparan sulfate-mediated FGF signaling.  |  Sreekumar, A., et al. 2024. Cancer Cell. 42: 52-69.e7. PMID: 38065100
12. Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells.  |  Habuchi, H., et al. 1998. J Biol Chem. 273: 9208-13. PMID: 9535912

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HS6ST1 Antibody (C-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HS6ST1 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HS6ST1 Antibody (C-9) Specifications

Alternate Product Name: HS6ST1

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 52-79

Epitope Target: HS6ST1

Epitope Species: human

Product Reactivity/Detection: HS6ST1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HS6ST1 Antibody (C-9): P: Blocking Peptide