HSC70/HSP70 Antibody (W27): sc-24

HSP 70/HSC 70 Antibody (W27) is a mouse monoclonal IgG2a λ light chain antibody that detects HSP 70/HSC 70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). HSP 70/HSC 70 (W27) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The HSP 70 family, which includes HSP 70, HSC 70, GRP 75, and GRP 78, plays crucial roles in cellular function, particularly as molecular chaperones that assist in proper folding of nascent polypeptide chains and assembly of multi-protein complexes. Notably, HSP 70 is induced in response to heat stress, highlighting its importance in cellular protection against stress-induced damage. HSP 70 and HSC 70 are primarily located in the cytosol and nucleus, where these proteins facilitate polypeptide translocation across membranes and help prevent misfolded protein accumulation, thereby maintaining cellular homeostasis. Additionally, GRP 78, localized in the endoplasmic reticulum, is essential for folding and translocation of secretory proteins, while GRP 75, found in the mitochondrial matrix, assists in translocation and folding of polypeptides of both nuclear and mitochondrial origin. The ability of HSP 70 family proteins to respond to cellular stress and assist in protein homeostasis underscores their significance in various physiological and pathological processes, making anti-HSP 70/HSC 70 antibody (W27) a valuable tool for researchers studying protein dynamics and stress responses in cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSC70/HSP70 Antibody (W27) has 525 citations in a variety of scientific publications.

Primary antibodies like Anti-HSC70/HSP70 Antibody (W27) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSC70/HSP70 Antibody (W27) Specifications

Alternate Product Name: heat shock protein A (Hsp70)

Product Clone Name: W27

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids:

Epitope Target: HSP 70

Epitope Species: human

Product Reactivity/Detection: HSP 70 and HSC 70

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 525 citations in various scientific publications

Available Conjugates/Forms for HSC70/HSP70 Antibody (W27): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent