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ABP1 Antibody (F-9): sc-398006

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Datasheets
  • ABP1 Antibody (F-9) is a mouse monoclonal IgG1 κ ABP1 antibody provided at 200 µg/ml
  • raised against amino acids 351-429 mapping within an internal region of ABP1 of human origin
  • ABP1 Antibody (F-9) is recommended for detection of ABP1 of human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-ABP1 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABP1 (F-9): sc-398006.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ABP1 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ABP1 Antibody (F-9) (see ordering information below).

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    SEE ALSO...

    ABP1 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ABP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ABP1 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ABP1, also known as amine oxidase, copper containing 1, kidney amine oxidase, and diamine oxidase, is a crucial enzyme belonging to the copper/topaquinone oxidase family. ABP1 plays a significant role in degrading biogenic amines such as putrescine, histamine, spermine, and spermidine, which are involved in various physiological processes, including allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and potentially apoptosis. ABP1′s presence in the extracellular space of the placenta and kidney highlights its importance in regulating female reproductive function, particularly during pregnancy. Notably, ABP1 consists of two isoforms due to alternative splicing, with isoform 1 being the common 751 amino acid form and isoform 2 containing an additional 19 amino acids between residues 619 and 637, which may influence functional properties and interactions within the cellular environment.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    ABP1 Antibody (F-9) References:

    1. Purification and identification of hog-kidney histaminase.  |  KAPELLER-ADLER, R. and MACFARLANE, H. 1963. Biochim Biophys Acta. 67: 542-65. PMID: 13962301
    2. [Intracellular distribution of diamine oxidase (histaminase) in the pig kidney].  |  VALETTE, G., et al. 1954. C R Seances Soc Biol Fil. 148: 1762-4. PMID: 14365019
    3. Semicarbazide sensitive amine oxidase overexpression has dual consequences: insulin mimicry and diabetes-like complications.  |  Stolen, CM., et al. 2004. FASEB J. 18: 702-4. PMID: 14977883
    4. Semicarbazide-sensitive amine oxidase in transgenic mice with diabetes.  |  Gokturk, C., et al. 2004. Biochem Biophys Res Commun. 325: 1013-20. PMID: 15541390
    5. Properties of copper-free pig kidney amine oxidase: role of topa quinone.  |  Mura, A., et al. 2006. FEBS Lett. 580: 4317-24. PMID: 16842785
    6. An important lysine residue in copper/quinone-containing amine oxidases.  |  Mura, A., et al. 2007. FEBS J. 274: 2585-95. PMID: 17433047
    7. Oxidation of p-dimethylaminomethylbenzylamine by pig kidney diamine oxidase. A new method for spectrophotometric assay.  |  Bardsley, WG., et al. 1972. Biochem J. 127: 875-9. PMID: 4627688
    8. 3-(p-hydroxyphenyl)propionic acid as a new fluorogenic reagent for amine oxidase assays.  |  Matsumoto, T., et al. 1984. Anal Biochem. 138: 133-6. PMID: 6428269
    9. Superoxide anion radical generation during the oxidation of various amines by diamine oxidase.  |  Silva, IJ., et al. 1996. Free Radic Res. 24: 167-75. PMID: 8728118

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ABP1 Antibody (F-9)

    sc-398006
    200 µg/ml
    $316.00

    ABP1 Antibody (F-9): m-IgG Fc BP-HRP Bundle

    sc-530657
    200 µg Ab; 10 µg BP
    $354.00

    ABP1 Antibody (F-9): m-IgGκ BP-HRP Bundle

    sc-524177
    200 µg Ab, 40 µg BP
    $354.00

    ABP1 Antibody (F-9): m-IgG1 BP-HRP Bundle

    sc-544185
    200 µg Ab; 20 µg BP
    $354.00

    ABP1 Antibody (F-9) AC

    sc-398006 AC
    500 µg/ml, 25% agarose
    $416.00

    ABP1 Antibody (F-9) HRP

    sc-398006 HRP
    200 µg/ml
    $316.00

    ABP1 Antibody (F-9) FITC

    sc-398006 FITC
    200 µg/ml
    $330.00

    ABP1 Antibody (F-9) PE

    sc-398006 PE
    200 µg/ml
    $343.00

    ABP1 Antibody (F-9) Alexa Fluor® 488

    sc-398006 AF488
    200 µg/ml
    $357.00

    ABP1 Antibody (F-9) Alexa Fluor® 546

    sc-398006 AF546
    200 µg/ml
    $357.00

    ABP1 Antibody (F-9) Alexa Fluor® 594

    sc-398006 AF594
    200 µg/ml
    $357.00

    ABP1 Antibody (F-9) Alexa Fluor® 647

    sc-398006 AF647
    200 µg/ml
    $357.00

    ABP1 Antibody (F-9) Alexa Fluor® 680

    sc-398006 AF680
    200 µg/ml
    $357.00

    ABP1 Antibody (F-9) Alexa Fluor® 790

    sc-398006 AF790
    200 µg/ml
    $357.00

    Can KAO (F-9): sc-398006 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: jerojero
    Thank you for your question. Yes, KAO (F-9): sc-398006 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Satisfactory Western blot data of KAO expressionSatisfactory Western blot data of KAO expression in human placenta and human kidney tissue extracts. -SCBT QC
    Date published: 2013-12-09
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    ABP1 Antibody (F-9) is rated 5.0 out of 5 by 1.
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