Muted Antibody (F-4): sc-393554
- Muted Antibody (F-4) is a mouse monoclonal IgG1 κ Muted antibody provided at 200 µg/ml
- raised against amino acids 64-187 mapping at the C-terminus of Muted of human origin
- Muted Antibody (F-4) is recommended for detection of Muted of human origin by WB, IP, IF and ELISA
- Anti-Muted Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Muted (F-4): sc-393554.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Muted Antibody (F-4) for WB applications. These reagents are now offered in bundles with Muted Antibody (F-4) (see ordering information below).
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Muted Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Muted protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Muted antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Muted protein plays a crucial role in the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a multisubunit complex essential for the formation of specialized organelles within the endosomal-lysosomal system, such as melanosomes and platelet-dense granules. This complex is composed of several coiled-coil-forming proteins, including Snapin, Pallidin, Cappuccino, Muted, BLOS1, BLOS2, and BLOS3, which exhibit varied localization within the cell, being found in cytoplasmic, peripheral membrane-bound, or vesicular membrane-anchored forms. Muted (mu) mouse serves as a valuable model for studying Hermansky-Pudlak Syndrome (HPS), a genetic disorder in humans characterized by hypopigmentation, bleeding disorders, and severe pulmonary complications leading to early mortality. Muted gene is expressed throughout the body, with particularly high levels in the brain, bone marrow, kidney, and liver, while lower expression is observed in skeletal muscle, highlighting its importance in various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Muted Antibody (F-4) References:
- The gene for the muted (mu) mouse, a model for Hermansky-Pudlak syndrome, defines a novel protein which regulates vesicle trafficking. | Zhang, Q., et al. 2002. Hum Mol Genet. 11: 697-706. PMID: 11912185
- Cappuccino, a mouse model of Hermansky-Pudlak syndrome, encodes a novel protein that is part of the pallidin-muted complex (BLOC-1). | Ciciotte, SL., et al. 2003. Blood. 101: 4402-7. PMID: 12576321
- [The effect of the characteristics of maternal psychological adaptation on the outcome of labor]. | Rymashevskiĭ, NV. and Volkov, AE. 1992. Akush Ginekol (Mosk). 15-8. PMID: 1292353
- Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). | Starcevic, M. and Dell'Angelica, EC. 2004. J Biol Chem. 279: 28393-401. PMID: 15102850
- Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex. | Gwynn, B., et al. 2004. Blood. 104: 3181-9. PMID: 15265785
- Normal lytic granule secretion by cytotoxic T lymphocytes deficient in BLOC-1, -2 and -3 and myosins Va, VIIa and XV. | Bossi, G., et al. 2005. Traffic. 6: 243-51. PMID: 15702992
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Muted Antibody (F-4) | sc-393554 | 200 µg/ml | $316.00 | |||
Muted Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-530493 | 200 µg Ab; 10 µg BP | $354.00 | |||
Muted Antibody (F-4): m-IgGκ BP-HRP Bundle | sc-523993 | 200 µg Ab, 40 µg BP | $354.00 | |||
Muted Antibody (F-4): m-IgG1 BP-HRP Bundle | sc-544087 | 200 µg Ab; 20 µg BP | $354.00 | |||
Muted Antibody (F-4) AC | sc-393554 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Muted Antibody (F-4) HRP | sc-393554 HRP | 200 µg/ml | $316.00 | |||
Muted Antibody (F-4) FITC | sc-393554 FITC | 200 µg/ml | $330.00 | |||
Muted Antibody (F-4) PE | sc-393554 PE | 200 µg/ml | $343.00 | |||
Muted Antibody (F-4) Alexa Fluor® 488 | sc-393554 AF488 | 200 µg/ml | $357.00 | |||
Muted Antibody (F-4) Alexa Fluor® 546 | sc-393554 AF546 | 200 µg/ml | $357.00 | |||
Muted Antibody (F-4) Alexa Fluor® 594 | sc-393554 AF594 | 200 µg/ml | $357.00 | |||
Muted Antibody (F-4) Alexa Fluor® 647 | sc-393554 AF647 | 200 µg/ml | $357.00 | |||
Muted Antibody (F-4) Alexa Fluor® 680 | sc-393554 AF680 | 200 µg/ml | $357.00 | |||
Muted Antibody (F-4) Alexa Fluor® 790 | sc-393554 AF790 | 200 µg/ml | $357.00 |