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PEPCK-C/PCK1 Antibody (A-3): sc-373972

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Datasheets
  • PEPCK-C/PCK1 Antibody (A-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-44 mapping at the N-terminus of PEPCK-C of human origin
  • recommended for detection of PEPCK-C of human origin by WB, IP, IF and ELISA
  • See PEPCK/PCK1/PCK2 (F-3): sc-271029 for PEPCK-C/PCK1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PEPCK-C/PCK1 Antibody (A-3) for WB applications. These reagents are now offered in bundles with PEPCK-C/PCK1 Antibody (A-3) (see ordering information below).

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    PEPCK-C Antibody (A-3) is a mouse monoclonal IgG2b kappa light chain antibody engineered to target the cytosolic form of Phosphoenolpyruvate carboxykinase (PEPCK-C), also known as PCK1. PEPCK-C monoclonal antibody (A-3) is raised against amino acids 1-44 at the N-terminus of human PEPCK-C, ensuring precise recognition and detection in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PEPCK-C is a crucial enzyme in the gluconeogenesis pathway, responsible for the synthesis of glucose from non-carbohydrate sources in liver cells, thereby maintaining blood glucose levels during fasting and metabolic stress. Structurally, PEPCK-C undergoes important post-translational modifications, including phosphorylation and acetylation, which regulate enzymatic activity and stability. These modifications are essential for the dynamic control of glucose production in response to hormonal signals like insulin and glucagon, which modulate PEPCK-C expression and activity. Dysregulation of these post-translational modifications is closely associated with metabolic disorders such as type 2 diabetes and obesity, highlighting PEPCK-C′s significance as a therapeutic target. PEPCK-C (A-3) antibody enables detailed studies of these regulatory mechanisms, supporting research aimed at understanding and treating insulin resistance and related metabolic conditions. By providing reliable and specific detection of PEPCK-C, anti-PEPCK-C antibody (A-3) serves as an indispensable tool for researchers investigating the molecular foundations of glucose metabolism and its associated diseases.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    PEPCK-C/PCK1 Antibody (A-3)

    sc-373972
    200 µg/ml
    $316.00

    PEPCK-C/PCK1 Antibody (A-3): m-IgGκ BP-HRP Bundle

    sc-535354
    200 µg Ab; 40 µg BP
    $354.00

    PEPCK-C/PCK1 Antibody (A-3): m-IgG2b BP-HRP Bundle

    sc-549400
    200 µg Ab; 10 µg BP
    $354.00

    What is the recommended fixation method for immunofluorescence staining with PEPCK-C (A-3): sc-373972 monoclonal antibody?

    Asked by: Professor Griffin
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Good specificity in WB in mouse transfectedGood specificity in WB in mouse transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-05-13
    Rated 5 out of 5 by from Produced beautiful immunofluorescence nucleolarProduced beautiful immunofluorescence nucleolar, nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-05-11
    Rated 5 out of 5 by from Robust signal in WB in human liver tissue extractRobust signal in WB in human liver tissue extract. -SCBT QC
    Date published: 2015-04-03
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    PEPCK-C/PCK1 Antibody (A-3) is rated 5.0 out of 5 by 3.
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