Rad9A (D2J4P) Rabbit mAb #14484
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The 9-1-1 complex interacts with DNA topoisomerase 2-binding protein 1 (TopBP1) in response to DNA damage, activating ATR and causing signal amplification through further recruitment of TopBP1 (4). The 9-1-1 complex interacts with DNA mismatch repair proteins MSH2, MSH3, and MSH6 to play a role in mismatch repair (5). During an error-free DNA damage tolerance process, the 9-1-1 complex cooperates with polyubiquitinated PCNA and Exo1 nuclease to support switching of the replicative polymerase to the undamaged template (6).
Research studies indicate that the two Rad9 paralogues (Rad9A and Rad9B) can both functionally complement one another and display distinct biological functions.
Specifically, Rad9B senses nucleolar stress and causes a delay in the cell cycle at G1/S phase (7).
- Broustas, C.G. and Lieberman, H.B. (2012) J Cell Biochem 113, 742-51.
- Kai, M. (2013) Biomolecules 3, 75-84.
- Bao, S. et al. (2004) Oncogene 23, 5586-93.
- Ohashi, E. et al. (2014) DNA Repair (Amst) 21, 1-11.
- Bai, H. et al. (2010) DNA Repair (Amst) 9, 478-87.
- Karras, G.I. et al. (2013) Mol Cell 49, 536-46.
- Pérez-Castro, A.J. and Freire, R. (2012) J Cell Sci 125, 1152-64.
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