PME-1 Antibody (F-6): sc-137162

PME-1 Antibody (F-6) is a mouse monoclonal IgG2a antibody that detects protein phosphatase methylesterase-1 (PME-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PME-1 plays a crucial role in cellular regulation by catalyzing the demethylation and inactivation of protein phosphatase 2A (PP2A), a key enzyme involved in growth inhibition and cell cycle arrest. Anti-PME-1 antibody (F-6) enables researchers to study how PME-1 modulates PP2A activity, which is vital for maintaining proper cellular function, as dysregulation of PP2A has been implicated in various diseases, including cancer. PME-1 is characterized by its conserved structure across species, containing a catalytic triad similar to that found in lipases, which underscores its evolutionary importance. Additionally, PME-1 undergoes post-translational modifications, such as carboxymethylation and demethylation, which are critical for regulating its activity and specificity. These modifications are influenced by electrostatic interactions at the active site, highlighting the intricate control mechanisms that govern PME-1′s function in cellular signaling pathways. PME-1 (F-6) antibody is an essential tool for researchers studying the dynamics of phosphatase regulation and its implications in health and disease.

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PME-1 Antibody (F-6) References:

1. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A.  |  Ogris, E., et al. 1999. J Biol Chem. 274: 14382-91. PMID: 10318862
2. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma.  |  Puustinen, P., et al. 2009. Cancer Res. 69: 2870-7. PMID: 19293187
3. PME-1 modulates protein phosphatase 2A activity to promote the malignant phenotype of endometrial cancer cells.  |  Wandzioch, E., et al. 2014. Cancer Res. 74: 4295-305. PMID: 24928782
4. Protein Phosphatase 2A and Its Methylation Modulating Enzymes LCMT-1 and PME-1 Are Dysregulated in Tauopathies of Progressive Supranuclear Palsy and Alzheimer Disease.  |  Park, HJ., et al. 2018. J Neuropathol Exp Neurol. 77: 139-148. PMID: 29281045
5. Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1.  |  Tang, S., et al. 2018. Life Sci. 213: 166-173. PMID: 30340029
6. Glycogen synthase kinase-3β suppresses the expression of protein phosphatase methylesterase-1 through β-catenin.  |  Jin, N., et al. 2019. Aging (Albany NY). 11: 9672-9688. PMID: 31714894
7. Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1.  |  Kauko, O., et al. 2020. J Biol Chem. 295: 4194-4211. PMID: 32071079
8. Inhibition of protein phosphatase methylesterase 1 dysregulates MAP kinase signaling and attenuates muscle cell differentiation.  |  Labuzan, SA., et al. 2020. Gene. 739: 144515. PMID: 32112987
9. Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice.  |  Staniszewski, A., et al. 2020. J Neurosci. 40: 4596-4608. PMID: 32341098
10. The luciferase-based in vivo protein-protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction.  |  Ando, S., et al. 2024. J Biol Chem. 300: 107277. PMID: 38588804

The preferred PME-1 antibody is PME-1 Antibody (B-12): sc-25278.