Sec61α Antibody (G-2): sc-393182

Sec61α Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sec61 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Sec61α antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sec61 alpha protein plays a crucial role in the translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane, a process essential for proper protein maturation and function. Sec61 alpha protein is part of the Sec61 complex, which acts as a channel through which newly synthesized proteins enter the ER, ensuring correct folding and modification before transport to final destinations within the cell. Evolutionary conservation of Sec61 alpha across species underscores a fundamental role in cellular biology. The Sec61 complex interacts with other key proteins, such as Sec62 and Sec63, which assist in the translocation process and are expressed in various human tissues, including the heart, brain, and liver. Sec61α (G-2) antibody effectively detects this vital protein, making Sec61α (G-2) antibody an invaluable tool for researchers studying protein synthesis and translocation mechanisms in mammalian cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sec61α Antibody (G-2) References:

1. Mammalian Sec61 is associated with Sec62 and Sec63.  |  Meyer, HA., et al. 2000. J Biol Chem. 275: 14550-7. PMID: 10799540
2. A protein-conducting channel in the endoplasmic reticulum.  |  Simon, SM. and Blobel, G. 1991. Cell. 65: 371-80. PMID: 1902142
3. Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum.  |  Erdmann, F., et al. 2011. EMBO J. 30: 17-31. PMID: 21102557
4. Ipomoeassin F Binds Sec61α to Inhibit Protein Translocation.  |  Zong, G., et al. 2019. J Am Chem Soc. 141: 8450-8461. PMID: 31059257
5. Aberrant expression of Sec61α in esophageal cancers.  |  Bachmann, K., et al. 2019. J Cancer Res Clin Oncol. 145: 2039-2044. PMID: 31197453
6. Phenylbutyrate rescues the transport defect of the Sec61α mutations V67G and T185A for renin.  |  Sicking, M., et al. 2022. Life Sci Alliance. 5: PMID: 35064074
7. The Pathway of a Transmembrane Helix Insertion into the Membrane Assisted by Sec61α Channel.  |  Gao, J. and Zhang, YW. 2024. Langmuir. 40: 16454-16462. PMID: 39046853
8. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane.  |  Görlich, D. and Rapoport, TA. 1993. Cell. 75: 615-30. PMID: 8242738
9. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes.  |  Rapoport, TA., et al. 1996. Annu Rev Biochem. 65: 271-303. PMID: 8811181
10. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.  |  Hanein, D., et al. 1996. Cell. 87: 721-32. PMID: 8929540
11. Identification of a human cDNA homologue to the Drosophila translocation protein 1 (Dtrp1).  |  Daimon, M., et al. 1997. Biochem Biophys Res Commun. 230: 100-4. PMID: 9020021
12. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex.  |  Beckmann, R., et al. 1997. Science. 278: 2123-6. PMID: 9405348

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sec61α Antibody (G-2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Sec61α Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sec61α Antibody (G-2) Specifications

Alternate Product Name: Sec61α

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 392-423

Epitope Target: Sec61α1

Epitope Species: human

Product Reactivity/Detection: Sec61α1 and Sec61α2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Sec61α Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin