TRIM Antibody (D-2): sc-365105
- TRIM Antibody (D-2) is a mouse monoclonal IgG1 κ TRIM antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 145-183 near the C-terminus of TRIM9TRIM of mouse origin
- TRIM Antibody (D-2) is recommended for detection of TRIM of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TRIM Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRIM (D-2): sc-365105.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRIM Antibody (D-2) for WB applications. These reagents are now offered in bundles with TRIM Antibody (D-2) (see ordering information below).
TRIM Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRIM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRIM (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRIM protein, known as the T-cell receptor interacting molecule, plays a crucial role as a transmembrane adaptor protein that associates with the TCR-CD3z complex in human T lymphocytes and T cell lines, facilitating effective T cell signaling and activation. TRIM protein is characterized by its type III transmembrane structure, which includes an 8-amino acid extracellular domain and an intracellular domain featuring four potential phosphorylation sites and eight tyrosine residues, three of which are implicated in SH2-mediated interactions with other signaling proteins. The human TRIM gene is located on chromosome 3q13, a region associated with susceptibility to rheumatoid arthritis, and is situated near the CD28, CD86, and CD80 genes, which encode important T-cell costimulatory molecules. TRIM is predominantly expressed in T-cells and natural killer cells, with localization at the cell membrane allowing direct interaction with CD3z and CD3e, thereby enhancing T cell receptor signaling and contributing to immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRIM Antibody (D-2) References:
- Genomic organization and chromosomal localization of the human gene encoding the T-cell receptor-interacting molecule (TRIM). | Hübener, C., et al. 2000. Immunogenetics. 51: 154-8. PMID: 10663578
- The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. | Kirchgessner, H., et al. 2001. J Exp Med. 193: 1269-84. PMID: 11390434
- Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. | Huynh, T., et al. 2001. Scand J Immunol. 54: 146-54. PMID: 11439161
- T cell receptor (TCR) interacting molecule (TRIM), a novel disulfide-linked dimer associated with the TCR-CD3-zeta complex, recruits intracellular signaling proteins to the plasma membrane. | Bruyns, E., et al. 1998. J Exp Med. 188: 561-75. PMID: 9687533
- High- and low-potency ligands with similar affinities for the TCR: the importance of kinetics in TCR signaling. | Kersh, GJ., et al. 1998. Immunity. 9: 817-26. PMID: 9881972
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM Antibody (D-2) | sc-365105 | 200 µg/ml | $316.00 | |||
TRIM Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-529310 | 200 µg Ab; 10 µg BP | $354.00 | |||
TRIM Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-522137 | 200 µg Ab, 40 µg BP | $354.00 | |||
TRIM Antibody (D-2): m-IgG1 BP-HRP Bundle | sc-543446 | 200 µg Ab; 20 µg BP | $354.00 | |||
TRIM Antibody (D-2) AC | sc-365105 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TRIM Antibody (D-2) HRP | sc-365105 HRP | 200 µg/ml | $316.00 | |||
TRIM Antibody (D-2) FITC | sc-365105 FITC | 200 µg/ml | $330.00 | |||
TRIM Antibody (D-2) PE | sc-365105 PE | 200 µg/ml | $343.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 488 | sc-365105 AF488 | 200 µg/ml | $357.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 546 | sc-365105 AF546 | 200 µg/ml | $357.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 594 | sc-365105 AF594 | 200 µg/ml | $357.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 647 | sc-365105 AF647 | 200 µg/ml | $357.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 680 | sc-365105 AF680 | 200 µg/ml | $357.00 | |||
TRIM Antibody (D-2) Alexa Fluor® 790 | sc-365105 AF790 | 200 µg/ml | $357.00 | |||
TRIM (D-2) Neutralizing Peptide | sc-365105 P | 100 µg/0.5 ml | $68.00 |