Apolipoprotein A I/APOA1 Antibody (B-10): sc-376818

apoA-I Antibody (B-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects apoA-I protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). apoA-I (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. apoA-I protein plays a crucial role in lipid metabolism, primarily functioning as a key component of high-density lipoprotein (HDL) in plasma, which is essential for reverse transport of cholesterol from peripheral tissues to liver for excretion. This process is vital for maintaining cholesterol homeostasis and preventing atherosclerosis, a condition characterized by buildup of fats, cholesterol, and other substances in and on artery walls. Additionally, apoA-I serves as a cofactor for lecithin cholesterol acyltransferase (LCAT), an enzyme that catalyzes formation of cholesteryl esters, further facilitating lipid transport and metabolism. The significance of apoA-I in cardiovascular health highlights anti-apoA-I antibody (B-10) applications in research and clinical studies focused on understanding lipid disorders and developing therapeutic strategies.

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Apolipoprotein A I/APOA1 Antibody (B-10) References:

1. High-density lipoprotein subclasses and apolipoprotein A-I.  |  Duriez, P. and Fruchart, JC. 1999. Clin Chim Acta. 286: 97-114. PMID: 10511287
2. Identification of an apolipoprotein A-I structural element that mediates cellular cholesterol efflux and stabilizes ATP binding cassette transporter A1.  |  Natarajan, P., et al. 2004. J Biol Chem. 279: 24044-52. PMID: 15051721
3. Apolipoprotein A-I-stimulated apolipoprotein E secretion from human macrophages is independent of cholesterol efflux.  |  Kockx, M., et al. 2004. J Biol Chem. 279: 25966-77. PMID: 15066991
4. The effects of scale: variation in the APOA1/C3/A4/A5 gene cluster.  |  Fullerton, SM., et al. 2004. Hum Genet. 115: 36-56. PMID: 15108119
5. Multiple rare alleles contribute to low plasma levels of HDL cholesterol.  |  Cohen, JC., et al. 2004. Science. 305: 869-72. PMID: 15297675
6. Identification and structural ramifications of a hinge domain in apolipoprotein A-I discoidal high-density lipoproteins of different size.  |  Maiorano, JN., et al. 2004. Biochemistry. 43: 11717-26. PMID: 15362856
7. Effect of pitavastatin on apolipoprotein A-I production in HepG2 cell.  |  Maejima, T., et al. 2004. Biochem Biophys Res Commun. 324: 835-9. PMID: 15474503
8. Conformation and lipid binding of the N-terminal (1-44) domain of human apolipoprotein A-I.  |  Zhu, HL. and Atkinson, D. 2004. Biochemistry. 43: 13156-64. PMID: 15476409
9. Apolipoprotein E isoforms and apolipoprotein AI protect from amyloid precursor protein carboxy terminal fragment-associated cytotoxicity.  |  Maezawa, I., et al. 2004. J Neurochem. 91: 1312-21. PMID: 15584908
10. ApoA-I mutations, L202P and K131del, in HDL from heterozygotes with low HDL-C.  |  Ljunggren, S., et al. 2014. Proteomics Clin Appl. 8: 241-50. PMID: 24273187
11. Structural determinants in ApoA-I amyloidogenic variants explain improved cholesterol metabolism despite low HDL levels.  |  Del Giudice, R., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 3038-3048. PMID: 28887204
12. AIBP and APOA-I synergistically inhibit intestinal tumor growth and metastasis by promoting cholesterol efflux.  |  Zhang, T., et al. 2019. J Transl Med. 17: 161. PMID: 31101050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Apolipoprotein A I/APOA1 Antibody (B-10) has 57 citations in a variety of scientific publications.

Primary antibodies like Anti-Apolipoprotein A I/APOA1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Apolipoprotein A I/APOA1 Antibody (B-10) Specifications

Alternate Product Name: apoA-I

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-267

Epitope Target: apoA-I

Epitope Species: human

Product Reactivity/Detection: apoA-I

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 57 citations in various scientific publications

Available Conjugates/Forms for Apolipoprotein A I/APOA1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin