karyopherin α6 Antibody (E-11): sc-390055

karyopherin α6 Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects karyopherin α6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin α6 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Karyopherin α6 plays a crucial role in nuclear import process, which is essential for cellular function as karyopherin α6 regulates transport of proteins containing nuclear localization signals (NLSs) into nucleus. This transport is vital for proper functioning of many cellular processes, including gene expression and cell cycle regulation. Karyopherin α6, along with karyopherin α1, serves as an adaptor for karyopherin β1, facilitating recognition and docking of NLS-containing proteins at nuclear pore complex (NPC). Structure of karyopherin α6 includes one importin β-binding (IBB) domain and ten armadillo (ARM) repeats, which are critical for binding and transporting substrates effectively. Proper functioning of karyopherin α6 is essential for maintaining cellular homeostasis, and dysregulation in karyopherin α6 activity can lead to various diseases, including cancer and neurodegenerative disorders.

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karyopherin α6 Antibody (E-11) References:

1. Ebola virus VP24 binds karyopherin alpha1 and blocks STAT1 nuclear accumulation.  |  Reid, SP., et al. 2006. J Virol. 80: 5156-67. PMID: 16698996
2. KPNA6 (Importin {alpha}7)-mediated nuclear import of Keap1 represses the Nrf2-dependent antioxidant response.  |  Sun, Z., et al. 2011. Mol Cell Biol. 31: 1800-11. PMID: 21383067
3. HNF1A-MODY Mutations in Nuclear Localization Signal Impair HNF1A-Import Receptor KPNA6 Interactions.  |  Fareed, FMA., et al. 2021. Protein J. 40: 512-521. PMID: 33459938
4. Kpna6 deficiency causes infertility in male mice by disrupting spermatogenesis.  |  Liu, N., et al. 2021. Development. 148: PMID: 34473250
5. KPNA6 is a Cofactor of ANP32A/B in Supporting Influenza Virus Polymerase Activity.  |  Yu, M., et al. 2022. Microbiol Spectr. 10: e0207321. PMID: 35044222
6. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
7. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
8. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
9. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
10. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
11. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin α6 Antibody (E-11) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin α6 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin α6 Antibody (E-11) Specifications

Alternate Product Name: karyopherin α6

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 51-104

Epitope Target: karyopherin α6

Epitope Species: human

Product Reactivity/Detection: karyopherin α6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for karyopherin α6 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred karyopherin α1/6 antibody is karyopherin α1/6 Antibody (2D9): sc-101540.