3β-HSD Antibody (37-2): sc-100466

3β-HSD Antibody (37-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects 3β-HSD in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-3β-HSD antibody (37-2) is available in a non-conjugated form, specifically targeting 3β-HSD and its isoform 3β-HSD2. 3 beta-hydroxysteroid dehydrogenase (3β-HSD), also referred to as HSD3B1 or HSDB3, is a bifunctional enzyme essential for the synthesis of all classes of hormonal steroids, making 3β-HSD critical for various physiological processes, including reproduction and metabolism. 3β-HSD is localized to the membrane of the endoplasmic reticulum and is predominantly expressed in tissues such as the skin and placenta. Type I 3β-HSD catalyzes the oxidative conversion of δ5-ene-3-beta-hydroxy steroids and various ketosteroids, which are vital steps in steroidogenesis. Defects in the gene encoding 3β-HSD can lead to serious health issues, including classic salt-wasting conditions, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS), and an increased risk of prostate cancer. Congenital deficiency of 3β-HSD activity can result in a critical depletion of steroid formation, posing a life-threatening risk in young children.

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3β-HSD Antibody (37-2) References:

1. The murine 3beta-hydroxysteroid dehydrogenase (3beta-HSD) gene family: a postulated role for 3beta-HSD VI during early pregnancy.  |  Peng, L., et al. 2002. Mol Cell Endocrinol. 187: 213-21. PMID: 11988330
2. Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3beta-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activity.  |  Thomas, JL., et al. 2002. J Biol Chem. 277: 42795-801. PMID: 12205101
3. Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase.  |  Thomas, JL., et al. 2003. J Biol Chem. 278: 35483-90. PMID: 12832414
4. The hormonal phenotype of Nonclassic 3 beta-hydroxysteroid dehydrogenase (HSD3B) deficiency in hyperandrogenic females is associated with insulin-resistant polycystic ovary syndrome and is not a variant of inherited HSD3B2 deficiency.  |  Carbunaru, G., et al. 2004. J Clin Endocrinol Metab. 89: 783-94. PMID: 14764797
5. YY1 binding within the human HSD3B2 gene intron 1 is required for maximal basal promoter activity: identification of YY1 as the 3beta1-A factor.  |  Foti, DM. and Reichardt, JK. 2004. J Mol Endocrinol. 33: 99-119. PMID: 15291746
6. Serine 124 completes the Tyr, Lys and Ser triad responsible for the catalysis of human type 1 3beta-hydroxysteroid dehydrogenase.  |  Thomas, JL., et al. 2004. J Mol Endocrinol. 33: 253-61. PMID: 15291757
7. Structure/function of human type 1 3beta-hydroxysteroid dehydrogenase: An intrasubunit disulfide bond in the Rossmann-fold domain and a Cys residue in the active site are critical for substrate and coenzyme utilization.  |  Thomas, JL., et al. 2007. J Steroid Biochem Mol Biol. 107: 80-7. PMID: 17624763
8. 3beta-HSD activates DHEA in the songbird brain.  |  Schlinger, BA., et al. 2008. Neurochem Int. 52: 611-20. PMID: 17643555
9. Human 3beta-hydroxysteroid dehydrogenase types 1 and 2: Gene sequence variation and functional genomics.  |  Wang, L., et al. 2007. J Steroid Biochem Mol Biol. 107: 88-99. PMID: 17689071
10. Association between polymorphisms in HSD3B1 and UGT2B17 and prostate cancer risk.  |  Park, JY., et al. 2007. Urology. 70: 374-9. PMID: 17826523
11. HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions.  |  Mao, TL., et al. 2008. Am J Surg Pathol. 32: 236-42. PMID: 18223326
12. 3β-HSD expression in the CNS of a manakin and finch.  |  Eaton, J., et al. 2018. Gen Comp Endocrinol. 256: 43-49. PMID: 28935582

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-3β-HSD Antibody (37-2) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-3β-HSD Antibody (37-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

3β-HSD Antibody (37-2) Specifications

Alternate Product Name: 3β-HSD

Product Clone Name: 37-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-374

Epitope Target: 3β-HSD

Epitope Species: human

Product Reactivity/Detection: 3β-HSD and 3β-HSD2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for 3β-HSD Antibody (37-2): N/A

The preferred 3β-HSD antibody is 3β-HSD Antibody (A-1): sc-515120.