p-ATP-citrate synthase Antibody (A-12): sc-374647

p-ATP-citrate synthase Antibody (A-12) is a mouse monoclonal IgG3 kappa light chain antibody that detects p-ATP-citrate synthase, specifically the Ser 455 phosphorylated form, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-ATP-citrate synthase (A-12) antibody is offered in a non-conjugated format, allowing for versatile experimental design. ATP-citrate synthase, also known as ATP-citrate lyase or citrate cleavage enzyme, is a crucial cytoplasmic homotetramer that plays a significant role in cellular metabolism by catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate. The production of acetyl-CoA is vital for various biosynthetic pathways, including the synthesis of fatty acids and cholesterol, which are essential for maintaining cellular structure and function. Additionally, ATP-citrate synthase is implicated in the biosynthesis of acetylcholine in nervous tissue, highlighting its importance in neurotransmission and overall brain health. The gene encoding ATP-citrate synthase is located on chromosome 17q12-q21, and understanding ATP-citrate synthase function and regulation is critical for elucidating metabolic disorders and developing therapeutic strategies.

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p-ATP-citrate synthase Antibody (A-12) References:

1. Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins.  |  Sato, R., et al. 2000. J Biol Chem. 275: 12497-502. PMID: 10777536
2. Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter.  |  Moon, YA., et al. 2002. Exp Mol Med. 34: 60-8. PMID: 11989980
3. The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes.  |  Berwick, DC., et al. 2002. J Biol Chem. 277: 33895-900. PMID: 12107176
4. Identification of multifunctional ATP-citrate lyase kinase as the alpha-isoform of glycogen synthase kinase-3.  |  Hughes, K., et al. 1992. Biochem J. 288 (Pt 1): 309-14. PMID: 1332698
5. ATP-citrate lyase deficiency in the mouse.  |  Beigneux, AP., et al. 2004. J Biol Chem. 279: 9557-64. PMID: 14662765
6. Reverse genetic characterization of cytosolic acetyl-CoA generation by ATP-citrate lyase in Arabidopsis.  |  Fatland, BL., et al. 2005. Plant Cell. 17: 182-203. PMID: 15608338
7. Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: a window on renal citrate metabolism.  |  Tosukhowong, P., et al. 2005. Int J Urol. 12: 140-4. PMID: 15733107
8. A new strategy for studying protein kinase B and its three isoforms. Role of protein kinase B in phosphorylating glycogen synthase kinase-3, tuberin, WNK1, and ATP citrate lyase.  |  Sale, EM., et al. 2006. Biochemistry. 45: 213-23. PMID: 16388597
9. Sequence of sites on ATP-citrate lyase and phosphatase inhibitor 2 phosphorylated by multifunctional protein kinase (a glycogen synthase kinase 3 like kinase).  |  Ramakrishna, S., et al. 1990. Biochemistry. 29: 7617-24. PMID: 2176822
10. Increased glycogen synthase kinase-3β and hexose-6-phosphate dehydrogenase expression in adipose tissue may contribute to glucocorticoid-induced mouse visceral adiposity.  |  Yan, C., et al. 2016. Int J Obes (Lond). 40: 1233-41. PMID: 27102048
11. Phosphorylation of sites 3 and 2 in rabbit skeletal muscle glycogen synthase by a multifunctional protein kinase (ATP-citrate lyase kinase).  |  Sheorain, VS., et al. 1985. J Biol Chem. 260: 12287-92. PMID: 3930492
12. ATP citrate-lyase and glycogen synthase kinase-3 beta in 3T3-L1 cells during differentiation into adipocytes.  |  Benjamin, WB., et al. 1994. Biochem J. 300 (Pt 2): 477-82. PMID: 7911658

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ATP-citrate synthase Antibody (A-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ATP-citrate synthase Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ATP-citrate synthase Antibody (A-12) Specifications

Alternate Product Name: ATP-CS

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Ser 455

Epitope Target: ATP-citrate synthase

Epitope Species: human

Product Reactivity/Detection: Ser 455 phosphorylated ATP-citrate synthase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-ATP-citrate synthase Antibody (A-12): P: Blocking Peptide

The preferred ATP-citrate synthase antibody is ATP-citrate synthase Antibody (5F8D11): sc-517267.