CEL Antibody (E-4): sc-377087

CEL Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects CEL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-CEL antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Carboxyl ester lipase (CEL), previously known as cholesterol esterase or bile salt-stimulated lipase, plays a crucial role in lipid metabolism by hydrolyzing a diverse range of substrates, including cholesteryl esters, phospholipids, lysophospholipids, ceramide, and tri-, di-, and mono-acylglycerols. CEL′s active site features a catalytic triad composed of serine, histidine, and aspartate, which is essential for enzymatic activity. CEL is predominantly produced in the pancreas and lactating mammary gland, but expression also occurs in the liver, macrophages, and vessel wall, highlighting CEL′s importance in various physiological processes. CEL (E-4) antibody has proven valuable in studying CEL′s involvement in the assembly and secretion of chylomicrons, a critical step in lipid transport and metabolism, which underscores CEL′s significance in maintaining lipid homeostasis and potential implications in atherosclerosis and other metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CEL Antibody (E-4) References:

1. Bile salt-stimulated carboxyl ester lipase influences lipoprotein assembly and secretion in intestine: a process mediated via ceramide hydrolysis.  |  Kirby, RJ., et al. 2002. J Biol Chem. 277: 4104-9. PMID: 11733511
2. Transcriptional regulation of the human carboxyl ester lipase gene in THP-1 monocytes: an E-box required for activation binds upstream stimulatory factors 1 and 2.  |  Bengtsson, SH., et al. 2002. Biochem J. 365: 481-8. PMID: 11945176
3. Characterization of a VNTR polymorphism in the coding region of the CEL gene.  |  Higuchi, S., et al. 2002. J Hum Genet. 47: 213-5. PMID: 12166660
4. Carboxyl ester lipase: structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis.  |  Hui, DY. and Howles, PN. 2002. J Lipid Res. 43: 2017-30. PMID: 12454261
5. Liver receptor homolog 1 controls the expression of carboxyl ester lipase.  |  Fayard, E., et al. 2003. J Biol Chem. 278: 35725-31. PMID: 12853459
6. Association between a polymorphism in the carboxyl ester lipase gene and serum cholesterol profile.  |  Bengtsson-Ellmark, SH., et al. 2004. Eur J Hum Genet. 12: 627-32. PMID: 15114370
7. Carboxyl ester lipase expression in macrophages increases cholesteryl ester accumulation and promotes atherosclerosis.  |  Kodvawala, A., et al. 2005. J Biol Chem. 280: 38592-8. PMID: 16166077
8. Carboxyl-ester lipase maturity-onset diabetes of the young is associated with development of pancreatic cysts and upregulated MAPK signaling in secretin-stimulated duodenal fluid.  |  Ræder, H., et al. 2014. Diabetes. 63: 259-69. PMID: 24062244
9. A carboxyl ester lipase (CEL) mutant causes chronic pancreatitis by forming intracellular aggregates that activate apoptosis.  |  Xiao, X., et al. 2017. J Biol Chem. 292: 7744. PMID: 28500240
10. Purification of carboxyl ester lipase from human pancreas and the amino acid sequence of the N-terminal region.  |  Wang, CS. 1988. Biochem Biophys Res Commun. 155: 950-5. PMID: 3421974
11. Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase.  |  Brejchova, K., et al. 2022. Food Chem. 388: 132983. PMID: 35486985
12. Molecular cloning and expression of rabbit pancreatic cholesterol esterase.  |  Colwell, NS., et al. 1993. Biochim Biophys Acta. 1172: 175-80. PMID: 8439557

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CEL Antibody (E-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CEL Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CEL Antibody (E-4) Specifications

Alternate Product Name: CEL

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 439-475

Epitope Target: CEL

Epitope Species: human

Product Reactivity/Detection: CEL long isoform

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CEL Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin