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COX5b Antibody (W10): sc-100523

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Datasheets
  • COX5b Antibody (W10) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 100 µg/ml
  • raised against recombinant COX5b of human origin
  • recommended for detection of COX5b of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for COX5b Antibody (W10). This work is in progress.

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COX5b Antibody (W10) is a mouse monoclonal IgG1 antibody that detects COX5b in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). COX5b is a crucial component of the cytochrome c oxidase (COX) family, which plays a vital role in cellular respiration by facilitating the final step of the electron transport chain. COX5b is located in the inner mitochondrial membrane, where COX5b participates in the conversion of oxygen and hydrogen into water, a process essential for maintaining the proton gradient necessary for ATP production. COX5b expression is particularly important under hypoxic conditions, as COX5b transcription is upregulated to enhance the efficiency of the COX apoenzyme, thereby ensuring that cells can adapt to varying oxygen levels. Anti-COX5b antibody (W10) provides reliable detection and analysis of COX5b, enabling researchers to explore COX5b′s role in mitochondrial function and cellular metabolism.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

COX5b Antibody (W10)

sc-100523
100 µg/ml
$333.00

I am using COX5b (W10): sc-100523 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good immunofluorescence cytoplasmic stainingGood immunofluorescence cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-03-20
Rated 3 out of 5 by from Band detected in WB using Hep G2 whole cell lysateBand detected in WB using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-01-17
Rated 4 out of 5 by from Publishable data in WB using human embryonicPublishable data in WB using human embryonic cell lines. -SCBT Publication Review
Date published: 2015-01-10
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COX5b Antibody (W10) is rated 3.7 out of 5 by 3.
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