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DD4 Antibody (WW3): sc-100526

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Datasheets
  • DD4 Antibody (WW3) is a mouse monoclonal IgG1 κ DD4 antibody, cited in 1 publications, provided at 100 µg/ml
  • raised against recombinant DD4 of human origin
  • recommended for detection of DD4 of human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DD4 Antibody (WW3). This work is in progress.

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DD4 Antibody (WW3) is a mouse monoclonal IgG1 kappa light chain antibody that detects DD4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DD4 antibody (WW3) is available as a non-conjugated format. DD4 protein, also known as AKR1C4, is a member of the aldo-keto reductase superfamily and plays a crucial role in steroid hormone and xenobiotic metabolism. Specifically, DD4 is involved in 3-keto-5-dihydrosteroid reduction, which is essential for steroid hormone activity regulation and environmental compound detoxification. This enzymatic activity maintains hormonal balance and protects against potentially harmful substances. While DD1 and DD2 are widely expressed in various tissues, DD4 mRNA expression is predominantly found in the liver, highlighting specialized function in hepatic metabolism. DD4′s ability to interact with NADPH as a cofactor further underscores importance in biochemical pathways that influence both drug metabolism and steroid hormone regulation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

DD4 Antibody (WW3) References:

  1. Characterization of a novel variant (S145C/L311V) of 3alpha-hydroxysteroid/dihydrodiol dehydrogenase in human liver.  |  Kume, T., et al. 1999. Pharmacogenetics. 9: 763-71. PMID: 10634139
  2. Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes.  |  Nishizawa, M., et al. 2000. Genes Cells. 5: 111-25. PMID: 10672042
  3. A novel dihydrodiol dehydrogenase in bovine liver cytosol: purification and characterization of multiple forms of dihydrodiol dehydrogenase.  |  Mizoguchi, T., et al. 1992. J Biochem. 112: 523-9. PMID: 1491006
  4. Human hepatic 3 alpha-hydroxysteroid dehydrogenase: possible identity with human hepatic chlordecone reductase.  |  Binstock, JM., et al. 1992. Biochem Biophys Res Commun. 187: 760-6. PMID: 1530633
  5. Enzymatic characterization of a novel bovine liver dihydrodiol dehydrogenase--reaction mechanism and bile acid dehydrogenase activity.  |  Nanjo, H., et al. 1995. Biochim Biophys Acta. 1244: 53-61. PMID: 7766669
  6. Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization.  |  Khanna, M., et al. 1995. Genomics. 25: 588-90. PMID: 7789999
  7. Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells.  |  Hara, A., et al. 1996. Biochem J. 313 (Pt 2): 373-6. PMID: 8573067
  8. Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA.  |  Shiraishi, H., et al. 1998. Biochem J. 334 (Pt 2): 399-405. PMID: 9716498

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DD4 Antibody (WW3)

sc-100526
100 µg/ml
$333.00

Can sc-100526: DD4 (WW3) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-100526: DD4 (WW3) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Specific for DD4 in Western BlotThis antibody detects over-expressed human DD4 in transfected lysate, sc-173413. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Robust immunoperoxidase cytoplasmic stainingRobust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-05-03
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DD4 Antibody (WW3) is rated 4.3 out of 5 by 3.
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