IDH3A Antibody (B-7): sc-514358

IDH3A Antibody (B-7) is a mouse monoclonal IgM antibody that detects IDH3A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IDH3A, or isocitrate dehydrogenase [NAD] subunit a (mitochondrial), is a crucial 366 amino acid protein encoded by the human gene IDH3A, belonging to the isocitrate and isopropylmalate dehydrogenases family. IDH3A plays a vital role in cellular metabolism, primarily located in the mitochondrion, where IDH3A functions as part of a heterooligomeric complex composed of subunits α, β, and γ in a 2:1:1 ratio. IDH3A serves as a key enzyme in the tricarboxylic acid cycle, catalyzing the conversion of isocitrate to 2-oxoglutarate, a critical organic acid necessary for the glutamine synthetase/glutamate synthase cycle, which is essential for ammonium assimilation. IDH3A is allosterically activated by ADP, which lowers the Km for isocitrate, thereby enhancing enzymatic activity and ensuring efficient energy production within the cell. With an ability to bind magnesium or manganese ions, IDH3A is integral to maintaining metabolic homeostasis, making anti-IDH3A antibody (B-7) an invaluable tool for researchers studying metabolic pathways and mitochondrial function.

The preferred IDH3A antibody is IDH3A Antibody (A-10): sc-398021.