IVD Antibody (A-8): sc-514240

IVD Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects IVD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-IVD antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IVD protein, or Isovaleryl-CoA dehydrogenase, mitochondrial, is a crucial 423 amino acid enzyme encoded by the human gene IVD, located in the mitochondrial matrix. IVD (A-8) monoclonal antibody plays a vital role in studying the catabolism of branched-chain amino acids by detecting proteins that catalyze the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA, a key step in the metabolic pathway that helps maintain energy homeostasis. Disruptions in the IVD gene can lead to the production of dysfunctional isoforms, resulting in isovaleric acidemia, a serious metabolic disorder characterized by the accumulation of toxic metabolites. There are two recognized forms of this condition: the acute neonatal form, which can lead to severe metabolic acidosis and rapid mortality, and a chronic form that presents with intermittent episodes of ketoacidosis. Understanding the function of IVD protein is essential for developing therapeutic strategies for managing these metabolic disorders, making anti-IVD antibody (A-8) a valuable tool for researchers studying its role in human health and disease.

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IVD Antibody (A-8) References:

1. Exon skipping in IVD RNA processing in isovaleric acidemia caused by point mutations in the coding region of the IVD gene.  |  Vockley, J., et al. 2000. Am J Hum Genet. 66: 356-67. PMID: 10677295
2. IVD authorized representatives.  |  Donawa, ME. 2003. Med Device Technol. 14: 19-21. PMID: 12774572
3. The variant human isovaleryl-CoA dehydrogenase gene responsible for type II isovaleric acidemia determines an RNA splicing error, leading to the deletion of the entire second coding exon and the production of a truncated precursor protein that interacts poorly with mitochondrial import receptors.  |  Vockley, J., et al. 1992. J Biol Chem. 267: 2494-501. PMID: 1310317
4. Establishment of a practical enzymatic assay method for determination of isovaleryl-CoA dehydrogenase activity using high-performance liquid chromatography.  |  Tajima, G., et al. 2005. Clin Chim Acta. 353: 193-9. PMID: 15698607
5. Functional analysis of acyl-CoA dehydrogenase catalytic residue mutants using surface plasmon resonance and circular dichroism.  |  Goetzman, ES., et al. 2006. Mol Genet Metab. 87: 233-42. PMID: 16376132
6. Isovaleric acidemia: new aspects of genetic and phenotypic heterogeneity.  |  Vockley, J. and Ensenauer, R. 2006. Am J Med Genet C Semin Med Genet. 142C: 95-103. PMID: 16602101
7. Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery.  |  De Angelis, PM., et al. 2006. Mol Cancer. 5: 20. PMID: 16709241
8. Joint analysis of IVD herniation and degeneration by rat caudal needle puncture model.  |  Cunha, C., et al. 2017. J Orthop Res. 35: 258-268. PMID: 26610284
9. Toward Patient Specific Models of Pediatric IVDs: A Parametric Study of IVD Mechanical Properties.  |  Pickering, E., et al. 2021. Front Bioeng Biotechnol. 9: 632408. PMID: 33659242
10. Clinical evidence requirements according to the IVDR 2017/746: practical tools and references for underpinning clinical evidence of IVD-MDs.  |  Charrière, K. and Pazart, L. 2023. Clin Chem Lab Med. 61: 1150-1157. PMID: 36919280
11. SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential.  |  Tamagawa, S., et al. 2024. Redox Biol. 71: 103091. PMID: 38412803
12. Structural organization of the human isovaleryl-CoA dehydrogenase gene.  |  Parimoo, B. and Tanaka, K. 1993. Genomics. 15: 582-90. PMID: 8468053

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IVD Antibody (A-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-IVD Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IVD Antibody (A-8) Specifications

Alternate Product Name: IVD

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 274-396

Epitope Target: IVD

Epitope Species: human

Product Reactivity/Detection: IVD, mitochondrial isoform1 and 2 precursor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for IVD Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin