KAT I Antibody (C-7): sc-374531
- KAT I Antibody (C-7) is a mouse monoclonal IgG1 κ KAT I antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 6-29 at the N-terminus of KAT I of human origin
- KAT I Antibody (C-7) is recommended for detection of KAT I isoforms 1, 2 and 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
- Anti-KAT I Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of KAT I (C-7): sc-374531.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for KAT I Antibody (C-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with KAT I Antibody (C-7) (see ordering information below).
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KAT I Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects KAT I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). KAT I (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KAT I, a member of the class-I pyridoxal-phosphate-dependent aminotransferase family, is primarily located in the cytoplasm and plays a crucial role in glutamine catabolism. KAT I catalyzes the transamination of L-kynurenine to produce kynurenic acid, a metabolite of tryptophan that exhibits neuroprotective and anticonvulsant properties. KAT I′s cellular location is significant due to involvement in synaptic transmission, which has been linked to various neurological disorders, including schizophrenia and Huntington′s disease. KAT I also participates in the metabolism of cysteine conjugates derived from certain halogenated alkenes and alkanes, leading to the formation of reactive metabolites. KAT I has three isoforms, with isoform 1 being the full-length protein, isoform 2 lacking amino acids 68-117, and isoform 3 missing amino acids 251-422. Based on sequence similarity, KAT I functions as a homodimer, further emphasizing importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KAT I Antibody (C-7) References:
- Kynurenine aminotransferase I (KATI) isoform gene expression in the rat brain: an in situ hybridization study. | Tamburin, M., et al. 1999. Neuroreport. 10: 61-5. PMID: 10094134
- Kynurenine aminotransferase I activity in human placenta. | Milart, P., et al. 2001. Placenta. 22: 259-61. PMID: 11170833
- A missense mutation in kynurenine aminotransferase-1 in spontaneously hypertensive rats. | Kwok, JB., et al. 2002. J Biol Chem. 277: 35779-82. PMID: 12145272
- Ontogenic changes of kynurenine aminotransferase I activity and its expression in the chicken retina. | Rejdak, R., et al. 2003. Vision Res. 43: 1513-7. PMID: 12782065
- Decreased expression of kynurenine aminotransferase-I (KAT-I) in the substantia nigra of mice after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment. | Knyihár-Csillik, E., et al. 2004. Neuroscience. 126: 899-914. PMID: 15207325
- Crystal structure of human kynurenine aminotransferase I. | Rossi, F., et al. 2004. J Biol Chem. 279: 50214-20. PMID: 15364907
- pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I. | Han, Q., et al. 2004. Eur J Biochem. 271: 4804-14. PMID: 15606768
- Effect of 6-hydroxydopamine treatment on kynurenine aminotransferase-I (KAT-I) immunoreactivity of neurons and glial cells in the rat substantia nigra. | Knyihár-Csillik, E., et al. 2006. Acta Neuropathol. 112: 127-37. PMID: 16788821
- Increased kynurenic acid levels and decreased brain kynurenine aminotransferase I in patients with Down syndrome. | Baran, H., et al. 1996. Life Sci. 58: 1891-9. PMID: 8637415
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KAT I Antibody (C-7) | sc-374531 | 200 µg/ml | $316.00 | |||
KAT I Antibody (C-7): m-IgG Fc BP-HRP Bundle | sc-525976 | 200 µg Ab; 10 µg BP | $354.00 | |||
KAT I Antibody (C-7) AC | sc-374531 AC | 500 µg/ml, 25% agarose | $416.00 | |||
KAT I Antibody (C-7) HRP | sc-374531 HRP | 200 µg/ml | $316.00 | |||
KAT I Antibody (C-7) FITC | sc-374531 FITC | 200 µg/ml | $330.00 | |||
KAT I Antibody (C-7) PE | sc-374531 PE | 200 µg/ml | $343.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 488 | sc-374531 AF488 | 200 µg/ml | $357.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 546 | sc-374531 AF546 | 200 µg/ml | $357.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 594 | sc-374531 AF594 | 200 µg/ml | $357.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 647 | sc-374531 AF647 | 200 µg/ml | $357.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 680 | sc-374531 AF680 | 200 µg/ml | $357.00 | |||
KAT I Antibody (C-7) Alexa Fluor® 790 | sc-374531 AF790 | 200 µg/ml | $357.00 | |||
KAT I (C-7) Neutralizing Peptide | sc-374531 P | 100 µg/0.5 ml | $68.00 |