LIMP II Antibody (D-3): sc-55570

LIMP II Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects LIMP II (D-3) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LIMP II (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LIMP II protein plays a crucial role in lysosomal function, particularly in trafficking and fusion of lysosomes with other cellular organelles, which is essential for maintaining cellular homeostasis and protein degradation. LIMP II protein is localized within lysosomes, which are vital for degradation of macromolecules and recycling of cellular components. Proper functioning of lysosomes is critical for various cellular processes, including autophagy and immune response, as lysosomes help eliminate pathogens and damaged organelles. Lysosomal integral membrane proteins, including LIMP II protein, are key markers for lysosomes and are instrumental in studies involving microscopy, cellular fractionation, and investigation of protein trafficking through secretory pathway. LIMP II (D-3) antibody enables researchers to gain valuable insights into lysosomal dynamics and their implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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LIMP II Antibody (D-3) References:

1. LIMP-2 is a receptor for lysosomal mannose-6-phosphate-independent targeting of beta-glucocerebrosidase.  |  Reczek, D., et al. 2007. Cell. 131: 770-83. PMID: 18022370
2. Disease-causing mutations within the lysosomal integral membrane protein type 2 (LIMP-2) reveal the nature of binding to its ligand beta-glucocerebrosidase.  |  Blanz, J., et al. 2010. Hum Mol Genet. 19: 563-72. PMID: 19933215
3. Expression of the transmembrane lysosomal protein SCARB2/Limp-2 in renin secretory granules controls renin release.  |  Lee, D., et al. 2012. Nephron Exp Nephrol. 122: 103-13. PMID: 23635510
4. Absence of the lysosomal protein Limp-2 attenuates renal injury in crescentic glomerulonephritis.  |  Lee, DH., et al. 2014. Immunol Cell Biol. 92: 400-8. PMID: 24394995
5. Lysosome sorting of β-glucocerebrosidase by LIMP-2 is targeted by the mannose 6-phosphate receptor.  |  Zhao, Y., et al. 2014. Nat Commun. 5: 4321. PMID: 25027712
6. LIMP-2 expression is critical for β-glucocerebrosidase activity and α-synuclein clearance.  |  Rothaug, M., et al. 2014. Proc Natl Acad Sci U S A. 111: 15573-8. PMID: 25316793
7. Role of LIMP-2 in the intracellular trafficking of β-glucosidase in different human cellular models.  |  Malini, E., et al. 2015. FASEB J. 29: 3839-52. PMID: 26018676
8. Lysosomal integral membrane protein LGP85 (LIMP-2) is ubiquitinated at the N-terminal cytoplasmic domain.  |  Fujimoto, K., et al. 2020. Biochem Biophys Res Commun. 524: 424-430. PMID: 32007273
9. All-Atom Molecular Dynamics Simulations of Polyethylene Glycol (PEG) and LIMP-2 Reveal That PEG Penetrates Deep into the Proposed CD36 Cholesterol-Transport Tunnel.  |  Dalhaimer, P. and Blankenship, KR. 2022. ACS Omega. 7: 15728-15738. PMID: 35571795
10. The Deficiency of SCARB2/LIMP-2 Impairs Metabolism via Disrupted mTORC1-Dependent Mitochondrial OXPHOS.  |  Zou, Y., et al. 2022. Int J Mol Sci. 23: PMID: 35955761

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIMP II Antibody (D-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-LIMP II Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIMP II Antibody (D-3) Specifications

Alternate Product Name: LIMP II

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 249-478

Epitope Target: LIMP II

Epitope Species: human

Product Reactivity/Detection: LIMP II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for LIMP II Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LIMP II antibody is LIMP II Antibody (D-4): sc-55571.