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MAN2A1 Antibody (D-5): sc-377204

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Datasheets
  • MAN2A1 Antibody (D-5) is a mouse monoclonal IgG1 κ MAN2A1 antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 584-805 mapping within an internal region of MAN2A1 of human origin
  • MAN2A1 Antibody (D-5) is recommended for detection of MAN2A1 of human origin by WB, IP, IF and ELISA
  • Anti-MAN2A1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MAN2A1 (D-5): sc-377204.
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for MAN2A1 Antibody (D-5) for WB applications. This reagent is now offered in a bundle with MAN2A1 Antibody (D-5) (see ordering information below).

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SEE ALSO...

MAN2A1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAN2A1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAN2A1 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAN2A1, also known as mannosidase alpha class 2A member 1, is a crucial single-pass type II membrane protein that resides in the cisternae of the Golgi apparatus, where MAN2A1 plays a vital role in glycoprotein modification. Specifically, MAN2A1 is involved in N-glycan processing, catalyzing the hydrolysis of terminal alpha-D-mannose residues in the oligosaccharide Man5(GlcNAc)3, a critical step in glycoprotein maturation. This enzymatic activity is essential for proper protein folding and function, as well as for overall cellular glycosylation, which impacts various biological functions, including cell signaling, immune response, and protein stability. MAN2A1′s ability to utilize zinc as a cofactor further underscores MAN2A1′s importance in cellular metabolism and highlights MAN2A1′s potential as a target for therapeutic interventions in diseases associated with glycosylation defects.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

MAN2A1 Antibody (D-5) References:

  1. A human embryonic kidney 293T cell line mutated at the Golgi alpha-mannosidase II locus.  |  Crispin, M., et al. 2009. J Biol Chem. 284: 21684-95. PMID: 19465480
  2. Alteration of N-glycans and expression of their related glycogenes in the epithelial-mesenchymal transition of HCV29 bladder epithelial cells.  |  Guo, J., et al. 2014. Molecules. 19: 20073-90. PMID: 25470275
  3. Targeting genomic rearrangements in tumor cells through Cas9-mediated insertion of a suicide gene.  |  Chen, ZH., et al. 2017. Nat Biotechnol. 35: 543-550. PMID: 28459452
  4. Mannose glycosylation is an integral step for NIS localization and function in human breast cancer cells.  |  Rathod, M., et al. 2019. J Cell Sci. 132: PMID: 31455607
  5. Functional Annotation of Genetic Loci Associated With Sepsis Prioritizes Immune and Endothelial Cell Pathways.  |  Le, KTT., et al. 2019. Front Immunol. 10: 1949. PMID: 31475010
  6. Imputed expression of schizophrenia-associated genes and cognitive measures in patients with schizophrenia.  |  Fabbri, C., et al. 2022. Mol Genet Genomic Med. 10: e1942. PMID: 35488718
  7. The Recurrent Liver MAN2A1-FER Oncoprotein Lacks Kinase Activity: Implications for the Use of Tyrosine Kinase Inhibitors.  |  Desaunay, M., et al. 2024. Cell Mol Gastroenterol Hepatol. 17: 667-669. PMID: 38141924
  8. Employing Multi-Omics Analyses to Understand Changes during Kidney Development in Perinatal Interleukin-6 Animal Model.  |  Panzade, G., et al. 2024. Cells. 13: PMID: 39404429
  9. Whole-exome sequencing reveals novel genomic signatures and potential therapeutic targets during the progression of rectal neuroendocrine neoplasm.  |  Xu, S., et al. 2024. Cell Death Dis. 15: 833. PMID: 39548061
  10. Integrated Multi-Level Investigation of Friend Leukemia Integration 1 Transcription Factor as a Novel Immune-Inflammatory Biomarker in Rheumatoid Arthritis: Bridging Bioinformatics, Clinical Cohorts, and Mechanistic Validation.  |  Zhang, M., et al. 2025. J Inflamm Res. 18: 3105-3123. PMID: 40059948

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MAN2A1 Antibody (D-5)

sc-377204
200 µg/ml
$316.00

MAN2A1 Antibody (D-5): m-IgG Fc BP-HRP Bundle

sc-526089
200 µg Ab; 10 µg BP
$354.00

MAN2A1 Antibody (D-5) AC

sc-377204 AC
500 µg/ml, 25% agarose
$416.00

MAN2A1 Antibody (D-5) HRP

sc-377204 HRP
200 µg/ml
$316.00

MAN2A1 Antibody (D-5) FITC

sc-377204 FITC
200 µg/ml
$330.00

MAN2A1 Antibody (D-5) PE

sc-377204 PE
200 µg/ml
$343.00

MAN2A1 Antibody (D-5) Alexa Fluor® 488

sc-377204 AF488
200 µg/ml
$357.00

MAN2A1 Antibody (D-5) Alexa Fluor® 546

sc-377204 AF546
200 µg/ml
$357.00

MAN2A1 Antibody (D-5) Alexa Fluor® 594

sc-377204 AF594
200 µg/ml
$357.00

MAN2A1 Antibody (D-5) Alexa Fluor® 647

sc-377204 AF647
200 µg/ml
$357.00

MAN2A1 Antibody (D-5) Alexa Fluor® 680

sc-377204 AF680
200 µg/ml
$357.00

MAN2A1 Antibody (D-5) Alexa Fluor® 790

sc-377204 AF790
200 µg/ml
$357.00

Is this antibody suitable for flow cytometry if stained by intracellular staining? 

Asked by: Nucake
Thank you for your question. Unconjugated MAN2A1 Antibody (D-5) is only recommended for detection of MAN2A1 of human origin by WB, IP, IF and ELISA. We also offer Anti-MAN2A1 Antibody (D-5) conjugated to either AlexaFluor, phycoerythrin, or FITC for IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2024-03-21

What is the recommended starting dilution for Western blot for sc-377204: MAN2A1 (D-5) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from PhDThis antibody had great specificity against human MAN2A1 with a single band in the western blot assay. In addition, the binding affinity of the antibody with human MAN2A1 is very high. This antibody also had really low cross-reactivity against mouse, rat, rabbit origin MAN2A1. This antibody is suitable for IP, IF and ELISA assay.
Date published: 2020-12-29
Rated 1 out of 5 by from Not good for IFI used it for IF on PFA fixed HeLa cells at 1:50 and 1:100 dilution. It failed to stain golgi in both cases.
Date published: 2017-05-30
Rated 5 out of 5 by from good antibodyused this antibody for western blotting of whole cell lysate of a human fibroblast cell line. works well, only one clean band. Also works for IF in methanol fixed cells.
Date published: 2017-03-28
Rated 5 out of 5 by from Produced positive Western blot data of MAN2A1Produced positive Western blot data of MAN2A1 expression in Hep G2 and JAR whole cell lysates. -SCBT QC
Date published: 2014-09-14
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MAN2A1 Antibody (D-5) is rated 4.0 out of 5 by 4.
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