Ral A/B Antibody (E-7): sc-374582

Ral A/B Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ral A/B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ral A/B antibody (E-7) is available as the non-conjugated form. Ral A and Ral B are integral members of the Ras-related GTPase family, playing crucial roles in various cellular processes, including cell proliferation, differentiation, and survival. Ral A/B proteins are primarily located in the cytoplasm and can translocate to the plasma membrane upon activation, which is essential for their function in signaling pathways. Ral A/B proteins interact with specific downstream effectors, such as Ral BP-1, which contains a Rho-GTPase-activating domain, highlighting their importance in regulating cytoskeletal dynamics and cellular morphology. Additionally, Ral A and Ral B are activated by the unique nucleotide exchange factor Ral GDS and are deactivated by specific GTPase-activating proteins, distinguishing them from other Ras proteins. While Ral proteins do not induce transformed foci in recipient cells like their Ras counterparts, Ral A/B proteins are involved in critical signaling pathways, such as the Ras/Ral pathway that mediates phospholipase D activation by v-Src, further emphasizing their role in cellular signaling and potential implications in cancer biology.

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Ral A/B Antibody (E-7) References:

1. Ca2+/calmodulin stimulates GTP binding to the ras-related protein ral-A.  |  Wang, KL. and Roufogalis, BD. 1999. J Biol Chem. 274: 14525-8. PMID: 10329639
2. Localization of ral, a small Mr GTP-binding protein, to collecting duct cells in bovine and rat kidney.  |  Gupta, A., et al. 1991. Am J Physiol. 261: F1063-70. PMID: 1750519
3. Aurora-A overexpression enhances cell-aggregation of Ha-ras transformants through the MEK/ERK signaling pathway.  |  Tseng, YS., et al. 2009. BMC Cancer. 9: 435. PMID: 20003375
4. Cyclin D1 interacts and collaborates with Ral GTPases enhancing cell detachment and motility.  |  Fernández, RM., et al. 2011. Oncogene. 30: 1936-46. PMID: 21242975
5. Substrate specificity of clostridial glucosylating toxins and their function on colonocytes analyzed by proteomics techniques.  |  Zeiser, J., et al. 2013. J Proteome Res. 12: 1604-18. PMID: 23387933
6. Inactivation of RASA1 promotes melanoma tumorigenesis via R-Ras activation.  |  Sung, H., et al. 2016. Oncotarget. 7: 23885-96. PMID: 26993606
7. RalGPS2 Is Essential for Survival and Cell Cycle Progression of Lung Cancer Cells Independently of Its Established Substrates Ral GTPases.  |  O Santos, A., et al. 2016. PLoS One. 11: e0154840. PMID: 27149377
8. Functional PDF Signaling in the Drosophila Circadian Neural Circuit Is Gated by Ral A-Dependent Modulation.  |  Klose, M., et al. 2016. Neuron. 90: 781-794. PMID: 27161526
9. CDDO-Me Attenuates CA1 Neuronal Death by Facilitating RalBP1-Mediated Mitochondrial Fission and 4-HNE Efflux in the Rat Hippocampus Following Status Epilepticus.  |  Kim, JE., et al. 2022. Antioxidants (Basel). 11: PMID: 35624848
10. Identification and characterization of a calmodulin-binding domain in Ral-A, a Ras-related GTP-binding protein purified from human erythrocyte membrane.  |  Wang, KL., et al. 1997. J Biol Chem. 272: 16002-9. PMID: 9188503

The preferred Ral A antibody is Ral A Antibody (F-5): sc-374462.