NF-M Antibody (1A2): sc-20013

NF-M Antibody (1A2) is a mouse monoclonal IgG1 antibody that detects neurofilament-M (NF-M) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-M is a crucial component of the neuronal cytoskeleton, belonging to the intermediate filament family, and plays a significant role in maintaining the structural integrity of neurons. Dynamic nature is underscored by the presence of multiple phosphorylation sites that are targeted by various protein kinases, including protein kinase A, protein kinase C, and cyclin-dependent kinase 5, which are essential for regulating axon caliber and overall neuronal function. The phosphorylation state of NF-M is particularly important, as alterations in phosphorylation and metabolism are often linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making NF-M a valuable biomarker for studying these conditions. Anti-NF-M antibody (1A2) provides researchers with a powerful tool for investigating the role of neurofilaments in neuronal health and disease, facilitating a deeper understanding of the underlying mechanisms of neurodegeneration.

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NF-M Antibody (1A2) References:

1. Neurofilament proteins NF-L, NF-M and NF-H in brain of patients with Down syndrome and Alzheimer's disease.  |  Bajo, M., et al. 2001. Amino Acids. 21: 293-301. PMID: 11764410
2. Phosphorylation of the head domain of neurofilament protein (NF-M): a factor regulating topographic phosphorylation of NF-M tail domain KSP sites in neurons.  |  Zheng, YL., et al. 2003. J Biol Chem. 278: 24026-32. PMID: 12695506
3. Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M.  |  Kelly, BM., et al. 1992. J Cell Biol. 118: 397-410. PMID: 1321159
4. Association of the dopamine receptor interacting protein gene, NEF3, with early response to antipsychotic medication.  |  Strous, RD., et al. 2007. Int J Neuropsychopharmacol. 10: 321-33. PMID: 16734940
5. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein.  |  Napolitano, EW., et al. 1987. J Neurosci. 7: 2590-9. PMID: 2441012
6. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.  |  Levy, E., et al. 1987. Eur J Biochem. 166: 71-7. PMID: 3036526
7. Hybrid character of a large neurofilament protein (NF-M): intermediate filament type sequence followed by a long and acidic carboxy-terminal extension.  |  Geisler, N., et al. 1984. EMBO J. 3: 2701-6. PMID: 6439558
8. Expression of subtypes NF-L, NF-M and NF-H of neurofilament triplet proteins in the developing rat inner ear.  |  Nishizaki, K. and Anniko, M. 1995. ORL J Otorhinolaryngol Relat Spec. 57: 177-81. PMID: 7478449
9. Increasing neurofilament subunit NF-M expression reduces axonal NF-H, inhibits radial growth, and results in neurofilamentous accumulation in motor neurons.  |  Wong, PC., et al. 1995. J Cell Biol. 130: 1413-22. PMID: 7559762
10. Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M.  |  Veeranna,., et al. 1998. J Neurosci. 18: 4008-21. PMID: 9592082

The preferred NF-M antibody is NF-M Antibody (E-9): sc-398532.