robo2 Antibody (A-10): sc-376177
- robo2 Antibody (A-10) is a mouse monoclonal IgG1 κ robo2 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1281-1380 within a C-terminal cytoplasmic domain of robo2 of human origin
- robo2 Antibody (A-10) is recommended for detection of robo2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-robo2 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of robo2 (A-10): sc-376177.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for robo2 Antibody (A-10) for WB and IHC(P) applications. These reagents are now offered in bundles with robo2 Antibody (A-10) (see ordering information below).
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robo2 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects robo2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). robo2 Antibody (A-10) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. robo2 (A-10) antibody recognizes a protein that plays a crucial role in central nervous system development by mediating axon guidance, essential for proper neuronal connectivity. robo2 (A-10) monoclonal antibody targets a receptor for Slit protein, a key repellent guidance cue preventing axons from crossing the nervous system midline. This interaction establishes correct pathways for axonal growth, ensuring neurons connect with appropriate targets. robo2 expression regulation shows distinct patterns, with non-crossing axons exhibiting high levels and crossing axons showing decreased expression before crossing and increased expression afterward. This dynamic expression pattern enables precise axon navigation during development, highlighting robo2′s significance in neural circuit formation and nervous system integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
robo2 Antibody (A-10) References:
- The middle and the end: slit brings guidance and branching together in axon pathway selection. | Van Vactor, D. and Flanagan, JG. 1999. Neuron. 22: 649-52. PMID: 10230784
- astray, a zebrafish roundabout homolog required for retinal axon guidance. | Fricke, C., et al. 2001. Science. 292: 507-10. PMID: 11313496
- ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-β signalling. | Pinho, AV., et al. 2018. Nat Commun. 9: 5083. PMID: 30504844
- Robo2 contains a cryptic binding site for neural EGFL-like (NELL) protein 1/2. | Yamamoto, N., et al. 2019. J Biol Chem. 294: 4693-4703. PMID: 30700556
- Robo2 regulates synaptic oxytocin content by affecting actin dynamics. | Anbalagan, S., et al. 2019. Elife. 8: PMID: 31180321
- Disruption of Robo2-Baiap2 integrated signaling drives cystic disease. | Li, Q., et al. 2019. JCI Insight. 4: PMID: 31534052
- ROBO2 hampers malignant biological behavior and predicts a better prognosis in pancreatic adenocarcinoma. | Ding, C., et al. 2021. Scand J Gastroenterol. 56: 955-964. PMID: 34148491
- Robo2 Drives Target-Selective Peripheral Nerve Regeneration in Response to Glia-Derived Signals. | Murphy, PL., et al. 2022. J Neurosci. 42: 762-776. PMID: 34916258
- Conformational Change of the Hairpin-like-structured Robo2 Ectodomain Allows NELL1/2 Binding. | Miyaguchi, M., et al. 2022. J Mol Biol. 434: 167777. PMID: 35940226
- Robo2 promotes osteoblast differentiation and mineralization through autophagy and is activated by parathyroid hormone induction. | Yuan, N., et al. 2023. Ann Anat. 248: 152070. PMID: 36801365
- Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors. | Kidd, T., et al. 1998. Cell. 92: 205-15. PMID: 9458045
- The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans. | Zallen, JA., et al. 1998. Cell. 92: 217-27. PMID: 9458046
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
robo2 Antibody (A-10) | sc-376177 | 200 µg/ml | $316.00 | |||
robo2 Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-529830 | 200 µg Ab; 10 µg BP | $354.00 | |||
robo2 Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-522864 | 200 µg Ab, 40 µg BP | $354.00 | |||
robo2 Antibody (A-10) AC | sc-376177 AC | 500 µg/ml, 25% agarose | $416.00 | |||
robo2 Antibody (A-10) HRP | sc-376177 HRP | 200 µg/ml | $316.00 | |||
robo2 Antibody (A-10) FITC | sc-376177 FITC | 200 µg/ml | $330.00 | |||
robo2 Antibody (A-10) PE | sc-376177 PE | 200 µg/ml | $343.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 488 | sc-376177 AF488 | 200 µg/ml | $357.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 546 | sc-376177 AF546 | 200 µg/ml | $357.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 594 | sc-376177 AF594 | 200 µg/ml | $357.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 647 | sc-376177 AF647 | 200 µg/ml | $357.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 680 | sc-376177 AF680 | 200 µg/ml | $357.00 | |||
robo2 Antibody (A-10) Alexa Fluor® 790 | sc-376177 AF790 | 200 µg/ml | $357.00 |