ATP5F1 Antibody (C-12): sc-514419
- ATP5F1 Antibody (C-12) is a mouse monoclonal IgG1 κ ATP5F1 antibody, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 47-65 near the N-terminus of ATP5F1 of human origin
- ATP5F1 Antibody (C-12) is recommended for detection of ATP5F1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ATP5F1 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATP5F1 (C-12): sc-514419.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATP5F1 Antibody (C-12) for WB applications. These reagents are now offered in bundles with ATP5F1 Antibody (C-12) (see ordering information below).
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ATP5F1 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATP5F1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATP5F1 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATP5F1, also known as ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, plays a crucial role in cellular energy production by forming part of the F0 complex of mitochondrial ATP synthase, which is essential for ATP synthesis during oxidative phosphorylation. ATP5F1 is located in the mitochondrial membrane, where ATP5F1 contributes to the formation of a proton channel that allows protons to flow back into the mitochondrial matrix, driving the synthesis of ATP from adenosine diphosphate and inorganic phosphate. Proper functioning of ATP5F1 is vital for maintaining cellular energy levels, and any dysfunction can lead to metabolic disorders and mitochondrial diseases. ATP5F1 is encoded by a gene located on human chromosome 1, which spans approximately 260 million base pairs and comprises nearly 8% of the human genome, highlighting evolutionary significance and complexity of regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATP5F1 Antibody (C-12) References:
- Understanding ATP synthesis: structure and mechanism of the F1-ATPase (Review). | Leyva, JA., et al. 2003. Mol Membr Biol. 20: 27-33. PMID: 12745923
- Plasma mitochondrial coupling factor 6 in patients with acute myocardial infarction. | Ding, WH., et al. 2004. Hypertens Res. 27: 717-22. PMID: 15785006
- Intracellular signaling for vasoconstrictor coupling factor 6: novel function of beta-subunit of ATP synthase as receptor. | Osanai, T., et al. 2005. Hypertension. 46: 1140-6. PMID: 16230521
- Plasma level of mitochondrial coupling factor 6 increases in patients with coronary heart disease. | Chai, SB., et al. 2007. Circ J. 71: 693-7. PMID: 17456993
- Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6. | Javed, AA., et al. 1991. Gene. 97: 307-10. PMID: 1825642
- Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria. | Higuti, T., et al. 1991. Biochem Biophys Res Commun. 178: 793-9. PMID: 1830479
- Sequence analysis and mapping of a novel human mitochondrial ATP synthase subunit 9 cDNA (ATP5G3). | Yan, WL., et al. 1994. Genomics. 24: 375-7. PMID: 7698763
- Energy transduction in ATP synthase. | Elston, T., et al. 1998. Nature. 391: 510-3. PMID: 9461222
- Energy transduction in the F1 motor of ATP synthase. | Wang, H. and Oster, G. 1998. Nature. 396: 279-82. PMID: 9834036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP5F1 Antibody (C-12) | sc-514419 | 200 µg/ml | $316.00 | |||
ATP5F1 Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-531119 | 200 µg Ab; 10 µg BP | $354.00 | |||
ATP5F1 Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-524718 | 200 µg Ab, 40 µg BP | $354.00 | |||
ATP5F1 Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-544406 | 200 µg Ab; 20 µg BP | $354.00 | |||
ATP5F1 Antibody (C-12) AC | sc-514419 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ATP5F1 Antibody (C-12) HRP | sc-514419 HRP | 200 µg/ml | $316.00 | |||
ATP5F1 Antibody (C-12) FITC | sc-514419 FITC | 200 µg/ml | $330.00 | |||
ATP5F1 Antibody (C-12) PE | sc-514419 PE | 200 µg/ml | $343.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 488 | sc-514419 AF488 | 200 µg/ml | $357.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 546 | sc-514419 AF546 | 200 µg/ml | $357.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 594 | sc-514419 AF594 | 200 µg/ml | $357.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 647 | sc-514419 AF647 | 200 µg/ml | $357.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 680 | sc-514419 AF680 | 200 µg/ml | $357.00 | |||
ATP5F1 Antibody (C-12) Alexa Fluor® 790 | sc-514419 AF790 | 200 µg/ml | $357.00 | |||
ATP5F1 (C-12) Neutralizing Peptide | sc-514419 P | 100 µg/0.5 ml | $68.00 |